机构：[a]Vascular Surgery Department of Xuan Wu Hospital, Institute of Vascular Surgery, Capital Medical University, Beijing, China血管外科首都医科大学宣武医院[b]Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
Emerging evidence shows that Uhrf1 plays an important role in DNA damage response for maintaining genomic stability. Interestingly, Uhrf1 has a paralog Uhrf2 in mammals. Uhrf1 and Uhrf2 share similar domain architectures. However, the role of Uhrf2 in DNA damage response has not been studied yet. During the analysis of the expression level of Uhrf2 in different tissues, we found that Uhrf2 is highly expressed in aorta and aortic vascular smooth muscle cells. Thus, we studied the role of Uhrf2 in DNA damage response in aortic vascular smooth muscle cells. Using laser microirradiation, we found that like Uhrf1, Uhrf2 was recruited to the sites of DNA damage. We dissected the functional domains of Uhrf2 and found that the TTD, PHD and SRA domains are important for the relocation of Uhrf2 to the sites of DNA damage. Moreover, depletion of Uhrf2 suppressed DNA damage-induced H2AX phosphorylation and DNA damage repair. Taken together, our results demonstrate the function of Uhrf2 in DNA damage response. (C) 2013 Elsevier Inc. All rights reserved.
第一作者机构：[a]Vascular Surgery Department of Xuan Wu Hospital, Institute of Vascular Surgery, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[b]Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
推荐引用方式(GB/T 7714)：
Tao Luo,Shijun Cui,Chunjing Bian,et al.Uhrf2 is important for DNA damage response in vascular smooth muscle cells[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2013,441(1):65-70.doi:10.1016/j.bbrc.2013.10.018.
APA：
Tao Luo,Shijun Cui,Chunjing Bian&Xiaochun Yu.(2013).Uhrf2 is important for DNA damage response in vascular smooth muscle cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,441,(1)
MLA：
Tao Luo,et al."Uhrf2 is important for DNA damage response in vascular smooth muscle cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 441..1(2013):65-70
