机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA[b]Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA[c]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA[d]Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China临床科室血管科首都医科大学附属安贞医院[e]Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Nebraska, USA[f]VA Nebraska Western-Iowa Health Care System, VA Medical Center, Nebraska, USA[g]Department of Radiation Oncology, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA[h]Division of Oncology and Hematology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA[i]Department of Psychology, University of Nebraska-Lincoln, Nebraska, USA[j]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USA[k]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA
Human induced pluripotent stem cells (iPSCs) have unlimited proliferation capability and potential to differentiate into all somatic cells. Their derivatives contain patients' genetic information and can model many diseases. Additionally, derivatives of patient-specific iPSCs induce minimal immune rejection in vivo. With this unique combination of properties, iPSCs open the avenue to personalized medicine including personalized drug screening, toxicity test, cell therapy and tissue engineering. However, the further advance of iPSC-based personalized medicine is currently limited by the difficulty to generate iPSCs for large populations and at affordable cost. We here report a low-cost device to address this challenge. The device allows the entire bioprocess for generating high quality and quantity of iPSCs for one patient to be done automatically within a closed conical tube without cell passaging. Additionally, iPSCs can be further differentiated into somatic cells in the device. Thus, the device also allows integrated iPSCs generation, expansion and differentiation to produce any somatic cell types. This device can be made in large quantities at low cost for manufacturing iPSCs (and their derivatives in necessary) for large populations at affordable cost. It will significantly advance the iPSCs-based personalized medicine.
第一作者机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA
通讯作者：
通讯机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA[b]Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA[j]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USA[k]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA[*1]820 N 16th St, Lincoln, NE, 68588, USA.
推荐引用方式(GB/T 7714)：
Haishuang Lin,Qiang Li,Qian Du,et al.Integrated generation of induced pluripotent stem cells in a low-cost device[J].BIOMATERIALS.2019,189:23-36.doi:10.1016/j.biomaterials.2018.10.027.
APA：
Haishuang Lin,Qiang Li,Qian Du,Ou Wang,Zhanqi Wang...&Yuguo Lei.(2019).Integrated generation of induced pluripotent stem cells in a low-cost device.BIOMATERIALS,189,
MLA：
Haishuang Lin,et al."Integrated generation of induced pluripotent stem cells in a low-cost device".BIOMATERIALS 189.(2019):23-36
工程技术