机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China老年医学科首都医科大学宣武医院[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China
Oligodendrocyte progenitor cells (OPCs) hold great promise for treatment of dysmyelinating disorders, such as multiple sclerosis and cerebral palsy. Recent studies on generation of human OPCs mainly use human embryonic stem cells (hESCs) or neural stem cells (NSCs) as starter cell sources for the differentiation process. However, NSCs are restricted in availability and the present method for generation of oligodendrocytes (OLs) from ESCs often requires a lengthy period of time. Here, we demonstrated a protocol to efficiently derive OPCs from human induced pluripotent stem cells (hiPSCs) by forced expression of two transcription factors (2TFs), Sox10 and Olig2. With this method, PDGFR alpha(+) OPCs can be obtained in 14 days and O4(+) OPCs in 56 days. Furthermore, OPCs may be able to differentiate to mature OLs that could ensheath axons when co-cultured with rat cortical neurons. The results have implications in the development of autologous cell therapies.
基金：
the Stem Cell and Translation National Key Project (2016ZY05002449),
National Basic Research Program of China (2011CB965103, 2012CBA01307),
National Natural Science Foundation of China (81661130160, 81422014, 81561138004),
Beijing Municipal Natural Science Foundation (5142005),
Royal Society-Newton Advanced Fellowship (NA150482).
第一作者机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China
共同第一作者：
通讯作者：
通讯机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China
推荐引用方式(GB/T 7714)：
Pengyan Li,Mo Li,Xihe Tang,et al.Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2[J].SCIENCE CHINA-LIFE SCIENCES.2016,59(11):1131-1138.doi:10.1007/s11427-016-0165-3.
APA：
Pengyan Li,Mo Li,Xihe Tang,Shuyan Wang,Y. Alex Zhang&Zhiguo Chen.(2016).Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2.SCIENCE CHINA-LIFE SCIENCES,59,(11)
MLA：
Pengyan Li,et al."Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2".SCIENCE CHINA-LIFE SCIENCES 59..11(2016):1131-1138
生物学