机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[2]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[3]Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[4]Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China临床科室血管科首都医科大学附属安贞医院[5]Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[6]Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA[7]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA[8]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA
Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 x 10(7) cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation.
基金：
UNL-UNMC Science Engineering and Medicine (SEM) initiative grant
第一作者机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
通讯作者：
通讯机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[3]Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[7]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA[8]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA
推荐引用方式(GB/T 7714)：
Lin Haishuang,Du Qian,Li Qiang,et al.A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells[J].STEM CELL REPORTS.2018,11(2):454-469.doi:10.1016/j.stemcr.2018.07.001.
APA：
Lin, Haishuang,Du, Qian,Li, Qiang,Wang, Ou,Wang, Zhanqi...&Lei, Yuguo.(2018).A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells.STEM CELL REPORTS,11,(2)
MLA：
Lin, Haishuang,et al."A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells".STEM CELL REPORTS 11..2(2018):454-469
医学