机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of SooChow University, Suzhou 215004, China[2]Molecular Medicine Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[3]CHUL Research Center, Laval University, Quebec G1V 4G2, Canada[4]Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
The present study was designed to address the relationship between iron homeostasis and bone metabolism. Cultured hFOB 1.19 osteoblasts were incubated with selected concentrations of hepcidin (50, 100, and 200 nmol/L) for 20 h, harvested for extraction of total RNA and proteins, and the expression of ferroportin 1 was analyzed by RT-PCR and western blotting. The results showed the presence of ferroportin 1 expression in cultured hFOB 1.19 cells. Furthermore, the ferroportin 1 had a similar expression pattern in hFOB cells as in hepatocytes and enterocytes and was downregulated by hepcidin. Our data indicate that osteoblasts are target cells for hepcidin, suggest that hepcidin may have many more targets than previously recognized, and support the role of hepcidin in the development of osteoporosis.
基金：
three Jiangsu
provincial grants, BK2008165, RC2007100, and the
priority academic program development of Jiangsu
higher education institutions.
第一作者机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of SooChow University, Suzhou 215004, China[*1]Department of Orthopaedics, The Second Affiliated Hospital of SooChow University, Suzhou 215004, China.
通讯作者：
通讯机构：[*1]Department of Orthopaedics, The Second Affiliated Hospital of SooChow University, Suzhou 215004, China.[*2]Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
Youjia Xu ,Wei Zhang ,Peng Zhang ,et al.Downregulation of Ferroportin 1 Expression in hFOB1.19 Osteoblasts by Hepcidin[J].INFLAMMATION.2012,35(3):1058-61.doi:10.1007/s10753-011-9411-8.
APA：
Youjia Xu,,Wei Zhang,,Peng Zhang,,Li Xiao,,Aidong Wang,...&Kai Li.(2012).Downregulation of Ferroportin 1 Expression in hFOB1.19 Osteoblasts by Hepcidin.INFLAMMATION,35,(3)
MLA：
Youjia Xu,,et al."Downregulation of Ferroportin 1 Expression in hFOB1.19 Osteoblasts by Hepcidin".INFLAMMATION 35..3(2012):1058-61
