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Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases

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收录情况： ◇ SCIE

作者：
Ming Zhang[a,b,c] * ; Hongxia Zheng[d] ; Xiaolu Zhang[a,b,c] ; Xiaoli Tian[a,b,c] ; Shengdi Xu[a,b,c] ; You Liu[a,b] ; Shuyuan Jiang[a,b] ; Xiaolei Liu[a,b] ; Rui Shi[a,b] ; Kerui Gong[e] ; Shaochun Yan[a,b] ; He Wang[f] ; Guo Shao[a,b,c,*1] ; Zhanjun Yang[b,c,*2] ;
机构： [a]Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC [b]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Inner Mongolia, PRC [c]Beijing key laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, PR [d]Faculty of Foreign languages, Baotou Teacher’s College, Inner Mongolia, PRC [e]Department of Oral and Maxillofacial Surgery, University of California San Francsico, San Francisco, CA, USA [f]School of Health Science, University of Newcastle, Newcastle, Australia
出处：
DOI： 10.1080/15287394.2018.1504384
ISSN：

关键词： DNA methyltransferase HT22 cell differentiation nerve growth factor

摘要：
DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway.

基金：
This project was supported by the National Natural Science Foundation of China (Nos. 81460283,81660307,81760232), Scientific research foundation for returned overseas Chinese scholar (the Ministry of Education), Inner Mongolia Science Foundation (2015MS0322, 2016MS(LH)0307), Baotou Health Foundation (No.WSJJ2016008), Inner Mongolia Educational Research Foundation (No. NJZY17251), Baotou medical college Foundation (BYJJ-DF201602, BYJJ-YF201615, BSJJ201617, BYJJ-QM201633, BYJJ-QM201656, BYJJ201502), National Key R&D Program of China (2017YFC1308405).
语种：
外文
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PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类 | 3 区 环境科学与生态学
小类 | 3 区 环境科学 3 区 公共卫生、环境卫生与职业卫生 3 区 毒理学
最新[2025]版：
大类 | 4 区 医学
小类 | 3 区 环境科学 3 区 毒理学 4 区 公共卫生、环境卫生与职业卫生
JCR分区：
出版当年[2016]版：
Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Q2 ENVIRONMENTAL SCIENCES Q2 TOXICOLOGY
最新[2023]版：
Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Q3 ENVIRONMENTAL SCIENCES Q3 TOXICOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2016版] 出版当年五年平均 出版前一年[2015版] 出版后一年[2017版]

第一作者：
第一作者机构： [a]Biomedicine Research Center and Basic Medical College, Baotou Medical College, Inner Mongolia, PRC [b]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Inner Mongolia, PRC [c]Beijing key laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, PR
通讯作者：
通讯机构： [*]Biomedicine Research Center, Basic Medical College and Baotou medical college of Neuroscience institute, Baotou Medical College, Inner Mongolia, PRC
推荐引用方式(GB/T 7714)：
Ming Zhang,Hongxia Zheng,Xiaolu Zhang,et al.Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases[J].JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES.2018,81(21):1116-1122.doi:10.1080/15287394.2018.1504384.
APA：
Ming Zhang,Hongxia Zheng,Xiaolu Zhang,Xiaoli Tian,Shengdi Xu...&Zhanjun Yang.(2018).Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases.JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES,81,(21)
MLA：
Ming Zhang,et al."Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases".JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES 81..21(2018):1116-1122

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