机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China神经外科首都医科大学宣武医院[2]Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China[3]Department of Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China[4]China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, China中美神经科学研究所首都医科大学宣武医院
Background: We showed previously that 2-(2-benzofuranyl)-2-imidazoline (2-BFI), a ligand to type 2 imidazoline receptor (I2R) exerts neuroprotective effects in ischemia stroke via an unknown mechanism. The present study was to investigate whether 2-BFI can protect the neurovascular unit (NVU) using a rat model of 90 min focal cerebral ischemia. Methods: Rats were randomly divided into three groups: thesham-operated group; the vehicle control group and the 2-BFI group which received 2-BFI (3 mg/kg) immediately after the start of middle cerebralartery occlusion (MCAO). Neurological deficit score, infarct size, apoptosis level, brain water content and Evans Blue extravasation were assessed at 24 h after stroke. Expressions of occludin and zonula occludens 1 (ZO-1), collagen IV, aquaporin-4 (AQP-4), matrix metalloproteinase-9 (MMP-9) and MMP-2 were assessed by Western blotting. Results: 2-BFI treatment was associated with significant improvement of neurological performance and decreased infarct volume at 24 h after stroke. Apoptosis level reduced significantly by 2-BFI compared to the vehicle group (34.3 +/- 5.4% vs 56.1 +/- 7.9%, p < 0.05). Significant decreased of brain water content (79.5 +/- 2.6% vs 84.62 +/- 2%, p < 0.05) and Evans Blue extravasation (1.2 +/- 0.5 vs 2.5 +/- 0.41 mu g/g, p < 0.05) of ipsilateral hemisphere was observed in 2-BFI group compared to vehicle group. Expressions of occludin, ZO-1 and collagen IV were significantly higher while MMP-9 level significantly lower in 2-BFI group. AQP-4 and MMP-2 showed no difference between 2-BFI and the vehicle groups. Conclusions: These results suggest that the neuroprotective effects of 2-BFI in acute ischemic brain damage are at least partly due to the drug's ability to improve the functions of NVU.
基金：
the China National Funds for Distinguished Young Scientists (81325007),
the Distinguished Professor of Cheung Kong Scholars Program (T2014251),
the National Natural Science Foundation of China (81571114),
the Wenzhou Municipal Sci-Tech Bureau Program (Y20120154 and Y20140686),
the National Natural Science Foundation of China Projects for International Cooperation and Exchange (81620108011).
第一作者机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China[2]Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
通讯作者：
通讯机构：[*1]Department of Neurology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Xue Yuan Xi Lu #109, 325027, Wenzhou, China[*2]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Chang Chun Street #2, 100000, Beijing, China.
推荐引用方式(GB/T 7714)：
Zheng Zhang ,Linlei Zhang ,Jiaou Chen ,et al.2-(2-Benzofuranyl)-2-Imidazoline Mediates Neuroprotection by Regulating the Neurovascular Unit Integrity in a Rat Model of Focal Cerebral Ischemia[J].JOURNAL OF STROKE & CEREBROVASCULAR DISEASES.2018,27(6):1481-1489.doi:10.1016/j.jstrokecerebrovasdis.2017.12.041.
APA：
Zheng Zhang,,Linlei Zhang,,Jiaou Chen,,Yungang Cao,,Man Qu,...&Xunming Ji.(2018).2-(2-Benzofuranyl)-2-Imidazoline Mediates Neuroprotection by Regulating the Neurovascular Unit Integrity in a Rat Model of Focal Cerebral Ischemia.JOURNAL OF STROKE & CEREBROVASCULAR DISEASES,27,(6)
MLA：
Zheng Zhang,,et al."2-(2-Benzofuranyl)-2-Imidazoline Mediates Neuroprotection by Regulating the Neurovascular Unit Integrity in a Rat Model of Focal Cerebral Ischemia".JOURNAL OF STROKE & CEREBROVASCULAR DISEASES 27..6(2018):1481-1489
