机构：[a]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China首都医科大学宣武医院低氧医学研究室[b]Center for Neuroscience Discovery, Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA[c]Emergency Department, Xuanwu Hospital, Capital Medical University, Beijing 100053, China急诊科首都医科大学宣武医院[d]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China神经外科首都医科大学宣武医院[e]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing 100053, China[f]Center for Stroke, Beijing Institute for Brain Disorder, Beijing 100069, China
Background and purpose: We tested the hypothesis that limb remote ischemic conditioning (LRIC) treatment promotes arteriogenesis and increases Notch signaling activity during stroke recovery. Methods: Adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO). LRIC was applied after the onset of focal ischemia (per-conditioning), followed by repeated short episodes of remote ischemia 24h after reperfusion (post-conditioning). Cerebral blood flow (CBF) was measured by Laser Doppler Flowmetry. Immunohistochemistry was used to reveal alpha-smooth muscle actin (alpha-SMA) immunopositive cells in the arteries of the brain. The cerebral angioarchitecture was visualized with a latex perfusion technique. Results: LRIC treatment significantly elevated local cerebral blood flow and increased arteriogenesis as indicated by increased arterial diameter and vascular smooth muscle cell proliferation in the ischemic brain. The increased arteriogenesis significantly correlated with the functional outcome after stroke. Furthermore, LRIC treatment unregulated the expressions of Notch1 and Notch intracellular domain (NICD) in arteries surrounding the ischemic area. Conclusion: These results suggest that the therapeutic effects of LRIC may involve the promotion of arteriogenesis during the recovery phase after focal cerebral ischemia and that Notch1 signaling seems to be an important player in limb remote ischemia-mediated arteriogenesis. (C) 2016 Published by Elsevier B.V.
基金：
the Natural Science Foundationof China (grant number 81573867),
China National Funds forDistinguished Youth Scientist (grant number 81325007),
Distin-guished Professor of Cheung Kong Scholars Program (grant numberT2014251)
Scientific Special Funding of Capital Health Devel-opment(grant number 2016 4-1032).
第一作者机构：[a]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China[b]Center for Neuroscience Discovery, Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA[e]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing 100053, China[f]Center for Stroke, Beijing Institute for Brain Disorder, Beijing 100069, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Xuanwu Hospital, Cap-ital Medical University, Chang Chun Road 45, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Changhong Ren,Sijie Li,Brian Wang,et al.Limb remote ischemic conditioning increases Notch signaling activity and promotes arteriogenesis in the ischemic rat brain[J].BEHAVIOURAL BRAIN RESEARCH.2018,340:87-93.doi:10.1016/j.bbr.2016.10.036.
APA：
Changhong Ren,Sijie Li,Brian Wang,Rongrong Hana,,Ning Li...&Xunming Ji.(2018).Limb remote ischemic conditioning increases Notch signaling activity and promotes arteriogenesis in the ischemic rat brain.BEHAVIOURAL BRAIN RESEARCH,340,
MLA：
Changhong Ren,et al."Limb remote ischemic conditioning increases Notch signaling activity and promotes arteriogenesis in the ischemic rat brain".BEHAVIOURAL BRAIN RESEARCH 340.(2018):87-93
