Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke
机构：[a]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China低氧医学研究室[b]Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, Texas, USA[c]Department of Neurosurgery, The Affiliated Hospital of Weifang Medical College, Weifang, Shandong, China[d]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China神经外科[e]Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing, China[f]Center of Stroke, Beijing Institute for Brain Disorder, Beijing, China[g]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing, China
Purpose: Limb remote ischemic per-conditioning or post-conditioning has been shown to be neuroprotective after cerebral ischemic stroke. However, the effect of combining remote per-conditioning with post-conditioning on ischemic/reperfusion injury as well as the underlying mechanisms are largely unexplored. Methods: Here, adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO). The limb ischemic stimuluswas immediately applied after onset of focal ischemia (per-conditioning), followed by repeated short episodes of remote ischemia 24 hr after reperfusion (post-conditioning). The infarct volume, motor function, and the expression of neuroglobin (Ngb) were measured at different durations after reperfusion. Results: We found that a single episode of limb remote per-conditioning afforded short-term protection, but combining repeated remote post-conditioning during the 14 days after reperfusion significantly ameliorated cerebral ischemia/reperfusion injury. Interestingly, we also found that ischemic per-and post-conditioning significantly increased expression of Ngb, an oxygenbinding globin protein that has been demonstrated to be neuroprotective against stroke, at peri-infarct regions from day 1 to day 14 following ischemia/reperfusion. Conclusion: Our results suggest that the conventional per-conditioning combined with post-conditioning may be used as a novel neuroprotective strategy against ischemia-reperfusion injury, and Ngb seems to be one of the important players in limb remote ischemia-mediated neuroprotection.
第一作者机构：[a]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China[b]Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, Texas, USA[f]Center of Stroke, Beijing Institute for Brain Disorder, Beijing, China[g]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing, China
通讯作者：
通讯机构：[*1]Xuanwu Hospital, Capital Medical University[*1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Chang Chun Road 45, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Changhong Ren,Pengcheng Wang,Brian Wang,et al.Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke[J].RESTORATIVE NEUROLOGY AND NEUROSCIENCE.2015,33(3):369-379.doi:10.3233/RNN-140413.
APA：
Changhong Ren,Pengcheng Wang,Brian Wang,Ning Li,Weiguang Li...&Xunming Ji.(2015).Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke.RESTORATIVE NEUROLOGY AND NEUROSCIENCE,33,(3)
MLA：
Changhong Ren,et al."Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke".RESTORATIVE NEUROLOGY AND NEUROSCIENCE 33..3(2015):369-379
