机构：[1]Cerebrovascular Diseases Research Institute, Xuanwu Hospital of Capital Medical University, Beijing 100053首都医科大学?脑血管病研究所首都医科大学宣武医院[2]China?America Joint Institute of Neuroscience, Xuanwu Hospital of Capital Medical University, Beijing 100053首都医科大学宣武医院[3]Department of Neurosurgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730[4]Department of Neurosurgery, The Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, P.R. China
MicroRNAs (miRNAs) regulate biogenesis and disease development by targeting numerous mRNAs. miRNA (miR)-124 and its direct target, inhibitor of apoptosis-stimulating protein of p53 (iASPP), may be involved in tumor development and progression. The aim of the present study was to explore the role of miR-124-targeted iASPP in glioma. The results demonstrated that miR-124 was aberrantly expressed in astrocytic glioma tissue and in the human glioblastoma cell lines U87 and U251. The expression of miR-124 was lower in astrocytic gliomas compared with normal brain (NB) tissues, with a more reduced expression in higher-grade tumors. In addition, several miR-124 loci (including miR-124-1, miR-124-2 and miR-124-3) were revealed to be more highly methylated in U87 cells compared with methylation levels in U251 cells and NB cells. Furthermore, the expression of iASPP was higher in high-grade astrocytic gliomas compared with low-grade astrocytic gliomas. miR-124 overexpression effectively inhibited U87 and U251 cell migration. In addition, miR-124 regulated cell viability and arrested the cell cycle at the G0/G1 phase in these two cell lines. miR-124 also reduced the expression levels of the cell cycle related genes iASPP, cyclin-dependent kinase (CDK)4, CDK6 and cyclin D1. Results from the present study indicated that expression of the miR-124 target gene iASPP may contribute to glioma development and progression.
基金：
This work was supported by The National Natural Science Foundation of China (grant nos. 81000504, 81471209 and 81641055) and The Beijing Natural Science Foundation (grant no. 7132112).
第一作者机构：[1]Cerebrovascular Diseases Research Institute, Xuanwu Hospital of Capital Medical University, Beijing 100053[2]China?America Joint Institute of Neuroscience, Xuanwu Hospital of Capital Medical University, Beijing 100053
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Beijing Tongren Hospital, Capital Medical University, 1 Dong Jiao Min Xiang, Beijing 100730, P.R. China[*2]Department of Neurosurgery, The Affiliated Hospital of Weifang Medical College, 2428 Yuhe Road, Weifang, Shandong 261031, P.R. China
推荐引用方式(GB/T 7714)：
XIANGRONG LIU,JUN KANG,SI SUN,et al.iASPP, a microRNA-124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability[J].MOLECULAR MEDICINE REPORTS.2018,17(1):1970-1978.doi:10.3892/mmr.2017.8097.
APA：
XIANGRONG LIU,JUN KANG,SI SUN,YUMIN LUO,XUNMING JI...&SHANGFENG ZHAO.(2018).iASPP, a microRNA-124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability.MOLECULAR MEDICINE REPORTS,17,(1)
MLA：
XIANGRONG LIU,et al."iASPP, a microRNA-124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability".MOLECULAR MEDICINE REPORTS 17..1(2018):1970-1978
