机构：[a]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Institute for Brain Disorders, Center of Alzheimer's Disease, Beijing 100053, China首都医科大学宣武医院[b]Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China[c]Department of Infectious Diseases, Chifeng City Hospital, Chifeng Clinical Medicine College of Inner Mongolia Medical University, Chifeng, Inner Mongolia Autonomous Region, 024000, China[d]Department of Geriatrics, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China老年医学科首都医科大学宣武医院
Cognitive deficits may occur after mild traumatic brain injury (mTBI), but effective treatment modalities are presently unavailable. Caloric restriction (CR) has beneficial effects on neurodegenerative diseases and brain injury. However, the underlying mechanisms have not yet been clearly defined. Therefore, the aim of the present study was to investigate the short-term effects of CR treatment on cognitive function in mice after mTBI. Forty-five 12-week-old C57/BL6 mice were subjected to closed-head mTBI using a weight drop device. The mice were then randomly divided into three groups according to their diet for 30 days: the normal calorie group (mTBI + NC group, n = 15), the caloric restriction group (mTBI + CR group, n = 15), and the high energy group (mTBI + HE group, n = 15). After 30 days, the Morris water maze test was performed to evaluate learning abilities. Nissl staining, immunohistochemistry, and western blotting were used to monitor pathological changes and changes in autophagy-associated proteins in the hippocampus. The average escape latency was significantly shorter in the mTBI + CR group than in the mTBI + NC and mTBI + HE groups, and the number of target platform crossings in the mTBI + CR group was significantly higher than in the other two groups. In the hippocampus, the expression of GFAP and mTOR was increased in the mTBI + HE group and decreased in the mTBI + CR group. Conversely, the expression of LC3B was decreased in the mTBI + HE group and increased in the mTBI + CR group. Our findings suggest that short-term CR after mTBI may ameliorate cognitive dysfunction induced by mTBI by increasing the level of autophagy and suppressing astrocyte activation.
基金：
Beijing Natural Science Foundation (7132033, 7132044, 7174310),
National Natural Science Foundation of China (81600927).
第一作者机构：[a]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Institute for Brain Disorders, Center of Alzheimer's Disease, Beijing 100053, China
通讯作者：
通讯机构：[a]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Institute for Brain Disorders, Center of Alzheimer's Disease, Beijing 100053, China[b]Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Yuan Liu,Rong Wang,Zhiwei Zhao,et al.Short-term caloric restriction exerts neuroprotective effects following mild traumatic brain injury by promoting autophagy and inhibiting astrocyte activation[J].BEHAVIOURAL BRAIN RESEARCH.2017,331:135-142.doi:10.1016/j.bbr.2017.04.024.
APA：
Yuan Liu,Rong Wang,Zhiwei Zhao,Wen Dong,Xu Zhang...&Lina Ma.(2017).Short-term caloric restriction exerts neuroprotective effects following mild traumatic brain injury by promoting autophagy and inhibiting astrocyte activation.BEHAVIOURAL BRAIN RESEARCH,331,
MLA：
Yuan Liu,et al."Short-term caloric restriction exerts neuroprotective effects following mild traumatic brain injury by promoting autophagy and inhibiting astrocyte activation".BEHAVIOURAL BRAIN RESEARCH 331.(2017):135-142
