机构：[1]Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China[2]Pittsburgh Institute of Brain Disorders & Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA, USA[3]Department of Gynecology and Obstetrics, Xuanwu Hospital, Capital Medical University, Beijing, China妇产科首都医科大学宣武医院[4]Division of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University, Pittsburgh, PA, USA[5]Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA[6]Department of Neurosurgery, General Hospital of PLA, Beijing, China
Scaffolds composed of extracellular matrix (ECM) are being investigated for their ability to facilitate brain tissue remodeling and repair following injury. The present study tested the hypothesis that the implantation of brain-derived ECM would attenuate experimental traumatic brain injury (TBI) and explored potential underlying mechanisms. TBI was induced in mice by a controlled cortical impact (CCI). ECM was isolated from normal porcine brain tissue by decellularization methods, prepared as a hydrogel, and injected into the ipsilesional corpus callosum and striatum 1 h after CCI. Lesion volume and neurological function were evaluated up to 35 d after TBI. Immunohistochemistry was performed to assess post-TBI white matter integrity, reactive astrogliosis, and microglial activation. We found that ECM treatment reduced lesion volume and improved neuro-behavioral function. ECM-treated mice showed less post-TBI neurodegeneration in the hippocampus and less white matter injury than control, vehicle-treated mice. Furthermore, ECM ameliorated TBI-induced gliosis and microglial pro-inflammatory responses, thereby providing a favorable microenvironment for tissue repair. Our study indicates that brain ECM hydrogel implantation improved the brain microenvironment that facilitates post-TBI tissue recovery. Brain ECM offers excellent biocompatibility and holds potential as a therapeutic agent for TBI, alone or in combination with other treatments.
基金：
the U.S. National Institutes of Health grants NS045048, NS095671, and NS095029),
National Natural Science Foundation of China grants (#30970939 and #81272804, #81301066, and #81271365),
Beijing Natural Science Foundation (#7132046 to),
Beijing Nova Program (#XX2013019 ).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|2 区医学
小类|3 区细胞与组织工程3 区医学：研究与实验3 区移植
最新[2023]版：
大类|4 区医学
小类|4 区细胞与组织工程4 区医学：研究与实验4 区移植
JCR分区：
出版当年[2015]版：
Q2MEDICINE, RESEARCH & EXPERIMENTALQ2TRANSPLANTATIONQ3CELL & TISSUE ENGINEERING
最新[2023]版：
Q2MEDICINE, RESEARCH & EXPERIMENTALQ2TRANSPLANTATIONQ3CELL & TISSUE ENGINEERING
第一作者机构：[1]Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China[2]Pittsburgh Institute of Brain Disorders & Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA, USA
共同第一作者：
通讯作者：
通讯机构：[*1]Pittsburgh Institute of Brain Disorders & Recovery, University of Pittsburgh, S507 Biomedical Science Tower, 3500 Terrace Street, Pittsburgh, PA 15213, USA.[*2]Department of Neurosurgery, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, China.
推荐引用方式(GB/T 7714)：
Yun Wu,Jiayin Wang,Yejie Shi,et al.Implantation of Brain-Derived Extracellular Matrix Enhances Neurological Recovery after Traumatic Brain Injury[J].CELL TRANSPLANTATION.2017,26(7):1224-1234.doi:10.1177/0963689717714090.
APA：
Yun Wu,Jiayin Wang,Yejie Shi,Hongjian Pu,Rehana K. Leak...&Ling Chen.(2017).Implantation of Brain-Derived Extracellular Matrix Enhances Neurological Recovery after Traumatic Brain Injury.CELL TRANSPLANTATION,26,(7)
MLA：
Yun Wu,et al."Implantation of Brain-Derived Extracellular Matrix Enhances Neurological Recovery after Traumatic Brain Injury".CELL TRANSPLANTATION 26..7(2017):1224-1234
