The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined with immunohistochemistry staining
机构：[1]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053神经内科首都医科大学宣武医院[2]Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030[3]Department of Hyperbaric Oxygen, Navy General Hospital, Beijing 100048, P.R. China
ADAM10 (a disintegrin and metalloprotease 10) is a member of the ADAMs family, which is key in the development of the nervous system, by regulating proliferation, migration, differentiation and survival of various cells, including axonal growth and myelination. Previous studies have investigated the embryonic or postnatal expression of ADAM10, however, detailed information regarding its cellular distribution in the adult stage, to the best of our knowledge, is not available. The present study investigated the expression pattern of the ADAM10 gene in the adult mouse central nervous system (CNS) using an ADAM10 complementary RNA probe for in situ hybridization (ISH). Immunohistochemical staining was used to identify the type of the ISH staining-positive cells with neuron- or astrocyte-specific antibodies. The results of the current study demonstrated that the ADAM10 gene was predominantly expressed in the neurons of the cerebral cortex, hippocampus, thalamus and cerebellar granular cells in adult mouse CNS.
基金：
the National Youth Fund (grant no. 81400931)
the Natural Foundation of Beijing (grant no. 7153715).
第一作者机构：[1]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053[*1]Department of Neurology, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, P.R. China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurology, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, P.R. China
推荐引用方式(GB/T 7714)：
ZHI-BAO GUO,YING-YING SU,YI-HUI WANG,et al.The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined with immunohistochemistry staining[J].MOLECULAR MEDICINE REPORTS.2016,14(3):2038-2044.doi:10.3892/mmr.2016.5501.
APA：
ZHI-BAO GUO,YING-YING SU,YI-HUI WANG,WEI WANG&DA-ZHI GUO.(2016).The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined with immunohistochemistry staining.MOLECULAR MEDICINE REPORTS,14,(3)
MLA：
ZHI-BAO GUO,et al."The expression pattern of Adam10 in the central nervous system of adult mice: Detection by in situ hybridization combined with immunohistochemistry staining".MOLECULAR MEDICINE REPORTS 14..3(2016):2038-2044
