Synucleinopathies and abnormalities in the nerves of the enteric nervous system are hypothesized to be involved in age-associated motility disorders. The aim of the present study was to investigate the expression of various antigens, including alpha-synuclein (Syn) and its post-translational modified forms, in the human colon at various ages. In addition, the study aimed to correlate the expression of Syn with neurodegeneration. Immunohistochemistry was used to detect the expression of neurofilament (NF), Syn, as well as its nitrated (N) form in the healthy colonic tissue of 12 young (34.08 +/- 5.12 years), 10 middle-aged (51.80 +/- 3.52 years), and 11 elderly (75.82 +/- 7.70 years) individuals. To the best of our knowledge, the current study is the first to demonstrate the presence of N-Syn in the colonic tissue. N-Syn was identified in the upper layer of the mucosa and submucosa layer. Furthermore, Syn (wild-type) was present in the mucosa and submucosa. The number of NF-positive neurons in the submucosal layer declined significantly with age (P<0.01). In addition, Syn and N-Syn significantly increased during aging (P<0.01). Furthermore, a negative correlation was identified between neuron number and synucleinopathies, indicating the abnormal accumulation of both wild-type Syn and N-Syn in the mucosa, submucosa, muscle layer and myenteric plexus. The present study demonstrates that the Syn pathology may be linked to colic neuronal degeneration during normal aging, and this link may cause functional deficits.