机构：[1]Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, PR China神经内科首都医科大学宣武医院[2]Department of Neurology, the Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou Province 550004, PR China.
Interaction of beta-amyloid peptide (A beta) with cell membranes is thought to be an initial step in Alzheimer's disease (AD). However, some data are controversial and the underlying mechanisms remain unclear. In this report, two populations of A beta were found in human SK-N-SH neuroblastoma cells. Notably, one of the A beta populations was tightly inserted into the plasma membrane whilst the other was only peripherally associated with it. Here we show that reducing membrane cholesterol decreased the number of membrane-embedded A beta s and increased the number of membrane-attached A beta s. We also found that cholesterol depletion inhibited A beta degradation and exacerbated A beta-mediated cytotoxicity. Our detailed and direct observations provide specific insights into the mechanism of A beta membrane-associated toxicity. (C) 2015 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
基金：
Beijing Postdoctoral Research Foundation, Chinese Postdoctoral Science Foundation,
the key project of the National Natural Science Foundation of China(30830045),
Seed Grant of International Alliance of Translational Neuroscience (PXM2014_014226_000006),
Scientific promoting project of Beijing Institute for Brain Disorders(BIBDPXM2014_014226_000016).
第一作者机构：[1]Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, PR China
通讯作者：
通讯机构：[1]Department of Neurology, Xuan Wu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, PR China
推荐引用方式(GB/T 7714)：
Ruiqin Liu ,Tian Tian,Jianping Jia.Characterization of the interactions between beta-amyloid peptide and the membranes of human SK-N-SH cells[J].FEBS LETTERS.2015,589(15):1929-1934.doi:10.1016/j.febslet.2015.05.035.
APA：
Ruiqin Liu,,Tian Tian&Jianping Jia.(2015).Characterization of the interactions between beta-amyloid peptide and the membranes of human SK-N-SH cells.FEBS LETTERS,589,(15)
MLA：
Ruiqin Liu,,et al."Characterization of the interactions between beta-amyloid peptide and the membranes of human SK-N-SH cells".FEBS LETTERS 589..15(2015):1929-1934
