Luteolin Isolated from the Medicinal Plant Elsholtzia rugulosa (Labiatae) Prevents Copper-Mediated Toxicity in beta-Amyloid Precursor Protein Swedish Mutation Overexpressing SH-SY5Y Cells
机构：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China[2]Xuanwu Hospital Capital Medical University, Beijing 100053, China首都医科大学宣武医院
Luteolin, a 3', 4', 5,7-tetrahydroxyflavone, is a plant flavonoid and pharmacologically active agent that has been isolated from several plant species. In the present study, the effects of luteolin obtained from the medicinal plant Elsholtzia rugulosa and the related mechanisms were examined in an Alzheimer's disease (AD) cell model. In this model, copper was used to exacerbate the neurotoxicity in beta-amyloid precursor protein Swedish mutation stably overexpressed SH-SY5Y cells (named "APPsw cells" for short). Based on this model, we demonstrated that luteolin increased cell viability, reduced intracellular ROS generation, enhanced the activity of SOD and reversed mitochondrial membrane potential dissipation. Inhibition of caspase-related apoptosis was consistently involved in the neuroprotection afforded by luteolin. Furthermore, it down-regulated the expression of A beta PP and lowered the secretion of A beta(1-42). These results indicated that luteolin from the Elsholtzia rugulosa exerted neroprotective effects through mechanisms that decrease A beta PP expression, lower A beta secretion, regulate the redox imbalance, preserve mitochondrial function, and depress the caspase family-related apoptosis.
基金：
National Natural Science Foundation of China (NO. 81073120)
Major Scientific and Technological Special Project for “Significant New Drugs Creation” (NO.2009ZX09302-003, 2009ZX09102-034, 2009ZX09102-123)
National Young Research Fund Project(2011CHX01)
International S&T Cooperation Program of China (NO. 2009DFA32010)
第一作者机构：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
通讯作者：
通讯机构：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
推荐引用方式(GB/T 7714)：
Rui Liu,Fanrui Meng,Li Zhang,et al.Luteolin Isolated from the Medicinal Plant Elsholtzia rugulosa (Labiatae) Prevents Copper-Mediated Toxicity in beta-Amyloid Precursor Protein Swedish Mutation Overexpressing SH-SY5Y Cells[J].MOLECULES.2011,16(3):2084-2096.doi:10.3390/molecules16032084.
APA：
Rui Liu,Fanrui Meng,Li Zhang,Ailin Liu,Hailin Qin...&Guanhua Du.(2011).Luteolin Isolated from the Medicinal Plant Elsholtzia rugulosa (Labiatae) Prevents Copper-Mediated Toxicity in beta-Amyloid Precursor Protein Swedish Mutation Overexpressing SH-SY5Y Cells.MOLECULES,16,(3)
MLA：
Rui Liu,et al."Luteolin Isolated from the Medicinal Plant Elsholtzia rugulosa (Labiatae) Prevents Copper-Mediated Toxicity in beta-Amyloid Precursor Protein Swedish Mutation Overexpressing SH-SY5Y Cells".MOLECULES 16..3(2011):2084-2096
