机构：[1]Department of Anatomy College of Basic Medicine, Beihua University, Jilin City, China[2]The Otolaryngological Department, China-Japan Union Hospital of Jilin University, Changchun, China[3]Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing, China神经内科首都医科大学宣武医院[4]Department of Hand Surgery, Affiliated Hospital of Beihua University, Jilin City, China
Sirt 1 plays a critical role in stress responses. We determined the deregulation of Sirt 1 activity, p53 acetylation, Bcl-2 expression, and mitochondria-dependent apoptosis in mouse osteoblast MC3T3-E1 cells which were exposed to H2O2. And then we investigated the protective role of Sirt 1 activator, Resveratrol (RSV), against the H2O2-induced apoptosis. Results demonstrated that Sirt 1 and Bcl-2 were inhibited, whereas p53 acetylation, Bax, and caspase 9 were promoted by H2O2, as was aggravated by the Sirt 1 inhibitor, EX-527. Instead, RSV inhibited the H2O2-induced both p53 acetylation and the caspase 9 activation, whereas ameliorated the H2O2-induced Bcl-2 inhibition and apoptosis. In conclusion, Sirt 1 was downregulated during the H2O2-induced apoptosis in MC3T3-E1 cells. And the chemical activation of Sirt 1 inhibited the H2O2-induced apoptosis via the downregulation of p53 acetylation. Our results suggest that Sirt 1 upregulation appears to be an important strategy to inhibit the oxidative stress-induced apoptosis.
基金：
the Health Department research plan of Jilin Province (No:20132030);
Changchun city of Jilin province science and technology plan projects (No:201339017).
第一作者机构：[1]Department of Anatomy College of Basic Medicine, Beihua University, Jilin City, China
通讯作者：
通讯机构：[4]Department of Hand Surgery, Affiliated Hospital of Beihua University, Jilin City, China
推荐引用方式(GB/T 7714)：
Na He,Xuewei Zhu,Wei He,et al.Resveratrol inhibits the hydrogen dioxide-induced apoptosis via Sirt 1 activation in osteoblast cells[J].BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY.2015,79(11):1779-1786.doi:10.1080/09168451.2015.1062712.
APA：
Na He,Xuewei Zhu,Wei He,Shiwei Zhao,Weiyan Zhao&Chunlei Zhu.(2015).Resveratrol inhibits the hydrogen dioxide-induced apoptosis via Sirt 1 activation in osteoblast cells.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,79,(11)
MLA：
Na He,et al."Resveratrol inhibits the hydrogen dioxide-induced apoptosis via Sirt 1 activation in osteoblast cells".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 79..11(2015):1779-1786
