机构：[1]China-America Joint Institute of Neuroscience, CAJIN, Xuanwu Hospital, Capital Medical University, Beijing, China首都医科大学宣武医院中美神经科学研究所[2]Neuroprotection Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts[3]Neuroprotection Research Laboratory, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts[4]Department of Neurosurgery, Affiliated Hospital of Weifang Medical College, Weifang, China[5]Center for Biological Electron Microscopy, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China[6]Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education, Ministry of Public Health, Peking University Health Science Center, Beijing 100191, China
Hypoxic preconditioning (HPC) elicits resistance to more drastic subsequent insults, which potentially provide neuroprotective therapeutic strategy, but the underlying mechanisms remain to be fully elucidated. Here, we examined the effects of HPC on synaptic ultrastructure in olfactory bulb of mice. Mice underwent up to five cycles of repeated HPC treatments, and hypoxic tolerance was assessed with a standard gasp reflex assay. As expected, HPC induced an increase in tolerance time. To assess synaptic responses, Western blots were used to quantify protein levels of representative markers for glia, neuron, and synapse, and transmission electron microscopy was used to examine synaptic ultrastructure and mitochondrial density. HPC did not significantly alter the protein levels of astroglial marker (GFAP), neuron-specific markers (GAP43, Tuj-1, and OMP), synaptic number markers (synaptophysin and SNAP25) or the percentage of excitatory synapses versus inhibitory synapses. However, HPC significantly affected synaptic curvature and the percentage of synapses with presynaptic mitochondria, which showed concomitant change pattern. These findings demonstrate that HPC is associated with changes in synaptic ultrastructure. (C) 2014 Wiley Periodicals, Inc.
基金：
National Natural Science Foundation of China;(Contract grant number: 81200915).
第一作者机构：[1]China-America Joint Institute of Neuroscience, CAJIN, Xuanwu Hospital, Capital Medical University, Beijing, China[2]Neuroprotection Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts[3]Neuroprotection Research Laboratory, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
共同第一作者：
通讯作者：
通讯机构：[*1]Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
YI LIU ,ZHISHAN SUN ,SHUFENG SUN ,et al.Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice[J].SYNAPSE.2015,69(1):7-14.doi:10.1002/syn.21777.
APA：
YI LIU,,ZHISHAN SUN,,SHUFENG SUN,,YUNXIA DUAN,,JINGFEI SHI,...&XUNMING JI.(2015).Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice.SYNAPSE,69,(1)
MLA：
YI LIU,,et al."Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice".SYNAPSE 69..1(2015):7-14
