机构：[1]School of Public Health, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, People’s Republic of China[2]Department of Neurology, Xuanwu Hospital, Capital Medical University, No.45 Changchun Street, Beijing 100053, People’s Republic of China神经内科首都医科大学宣武医院[3]Beijing Jishuitan Hospital, No.31 Xin Jie Kou Dong, Beijing 100035, People’s Republic of China
Genistein, the main soy isoflavone component, has received much attention for its potential multifunction. Here, we reported that in BV-2 cells, genistein significantly inhibited beta amyloid peptides 25-35 (A beta 25-35)-induced inflammatory response. The results indicated that A beta 25-35-stimulated BV-2 cells upregulated Toll-like receptors 2 and 4, Myd88, and IKK gene expression with the increasing expression of IL-6 and decreasing expression of TGF-beta and IL-10. Further, inhibiting TLR4 expression with small interfering RNA prevented the inflammatory response induced by A beta 25-35, indicating the key role of TLRs in A beta-mediated inflammation. Genistein pre-treated BV-2 cells showed less inflammatory response when exposed to A beta 25-35. These results suggested that A beta induced BV-2 cells inflammation though TLRs and genistein has an anti-inflammatory effect in vitro.
基金：
financial support provided by National Natural Science Foundation of China (no. 30972470)
National High Technology Research and Development Program of China(no. 2010AA023003)
Beijing Municipal Natural Science Foundation(no. 7102015)
第一作者机构：[1]School of Public Health, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]School of Public Health, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, People’s Republic of China[3]Beijing Jishuitan Hospital, No.31 Xin Jie Kou Dong, Beijing 100035, People’s Republic of China
推荐引用方式(GB/T 7714)：
Huan-Ling Yu,Xiao-Ying Li,Xin Zhou,et al.Beta Amyloid Peptide (25-35) Leading to Inflammation Through Toll-Like Receptors and the Anti-inflammatory Effect of Genistein in BV-2 Cells[J].JOURNAL OF MOLECULAR NEUROSCIENCE.2013,51(3):771-778.doi:10.1007/s12031-013-0063-z.
APA：
Huan-Ling Yu,Xiao-Ying Li,Xin Zhou,Lin-Hong Yuan,Wei-Wei Ma...&Rong Xiao.(2013).Beta Amyloid Peptide (25-35) Leading to Inflammation Through Toll-Like Receptors and the Anti-inflammatory Effect of Genistein in BV-2 Cells.JOURNAL OF MOLECULAR NEUROSCIENCE,51,(3)
MLA：
Huan-Ling Yu,et al."Beta Amyloid Peptide (25-35) Leading to Inflammation Through Toll-Like Receptors and the Anti-inflammatory Effect of Genistein in BV-2 Cells".JOURNAL OF MOLECULAR NEUROSCIENCE 51..3(2013):771-778
