机构：[1]National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China[2]Lab of Cell Secretion and Metabolism, Institute of Molecular Medicine, Peking University and National Center for Nanoscience and Technology, Beijing, 100871, China[3]Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China普通外科首都医科大学宣武医院[4]Graduate School of the Chinese Academy of Sciences, Beijing 100864, China
Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca2+ entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
基金：
the National Natural Science Foundation of China (Grant No. 30871225)
Beijing Municipal Science and Technology Commission (Grant No. 7121008)
the Ministry of Science and Technology (Grant No.SQ2011SF11B01041)
The Key Construction Program of the National “985” Project from the Department of Education of China to Peking University
第一作者机构：[1]National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China[4]Graduate School of the Chinese Academy of Sciences, Beijing 100864, China
通讯作者：
通讯机构：[1]National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
推荐引用方式(GB/T 7714)：
??Du Wen,Yanhong Xue,Kuo Liang,et al.Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells[J].PROTEIN & CELL.2012,3(8):618-626.doi:10.1007/s13238-012-2938-0.
APA：
??Du Wen,Yanhong Xue,Kuo Liang,Tianyi Yuan,Jingze Lu...&Liangyi Chen.(2012).Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells.PROTEIN & CELL,3,(8)
MLA：
??Du Wen,et al."Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells".PROTEIN & CELL 3..8(2012):618-626
生物学