机构：[1]Cerebrovascular Diseases Research Institute, Key Laboratory of Neurodegenerative Diseases of Ministry of Education and Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 100053 Beijing, People’s Republic of China神经外科神经变性病教育部重点实验室首都医科大学?脑血管病研究所首都医科大学宣武医院[2]Department of Neurosurgery, Beijing Tiantan Hospital, Capital University of Medical Sciences, 100050 Beijing, People’s Republic of China[3]Department of Neurosurgery, Beijing Tongren Hospital, Capital University of Medical Sciences, 100730 Beijing, People’s Republic of China
beta-Catenin, a core component of Wnt/beta-catenin signaling, has been shown to be a crucial factor in a broad range of tumors, while its role in glioma is not well understood. In this study, the expression of beta-catenin in astrocytic glioma tissues with different grade and human normal cerebral tissues was examined using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. We found a higher expression level of beta-catenin in astrocytic glioma patients with high grade in comparison with the normal controls. Additionally, siRNA was transfected into human U251 glioblastoma cells by liposome after the design of siRNA was confirmed to effectively inhibit the expression of beta-catenin by RT-PCR. Compared to the control siRNA group, siRNA-mediated knockdown of beta-catenin in human U251 cells inhibited cell proliferation, resulted in cell apoptosis, and arrested cell cycle in G (0)/G (1). Additionally, downregulation of beta-catenin decreased the expression level of cyclin D1, c-Myc and c-jun. Taken together, these results indicate that overexpression of beta-catenin may be an important contributing factor to glioma progression.
基金：
Postdoctoral Science Found (20080440423) of China and Clinical and Basic Cooperation Fund of Capital University of Medical Sciences (2006JL36).
第一作者机构：[1]Cerebrovascular Diseases Research Institute, Key Laboratory of Neurodegenerative Diseases of Ministry of Education and Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 100053 Beijing, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Cerebrovascular Diseases Research Institute, Key Laboratory of Neurodegenerative Diseases of Ministry of Education and Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 100053 Beijing, People’s Republic of China
推荐引用方式(GB/T 7714)：
Xiangrong Liu,Lei Wang,Shangfeng Zhao,et al.beta-Catenin overexpression in malignant glioma and its role in proliferation and apoptosis in glioblastma cells[J].MEDICAL ONCOLOGY.2011,28(2):608-14.doi:10.1007/s12032-010-9476-5.
APA：
Xiangrong Liu,Lei Wang,Shangfeng Zhao,Xunming Ji,Yumin Luo&Feng Ling.(2011).beta-Catenin overexpression in malignant glioma and its role in proliferation and apoptosis in glioblastma cells.MEDICAL ONCOLOGY,28,(2)
MLA：
Xiangrong Liu,et al."beta-Catenin overexpression in malignant glioma and its role in proliferation and apoptosis in glioblastma cells".MEDICAL ONCOLOGY 28..2(2011):608-14
