Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Combined with Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease
机构：[1]Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing , China老年医学科神经变性病教育部重点实验室首都医科大学宣武医院
Background: Biomarkers for neurodegenerative diseases are essential to facilitate disease diagnosis. Application of proteomics has greatly hastened the search for novel biomarkers. In this study, new potential biomarkers were discovered, and a diagnostic pattern was established for idiopathic Parkinson's disease (PD) by using proteomic technology. Methods: Serum proteins from PD patients and controls were captured by magnetic bead-based weak cation exchange. The molecular weight of the proteins in bead-binding fraction was detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Biomarker Wizard 3.1 and Biomarker Patterns Software were used for data analysis and constructing a model of biomarkers. A blinded testing set was used to validate the model. Results: A total of 17 discriminating m/z peaks related to PD were identified. The model based on the 5 biomarkers generated an excellent separation between PD and healthy controls with 98.36% for the sensitivity and 83.05% for the specificity. Blind test data demonstrated the model could recognize patients with PD with a sensitivity of 85.0% and a specificity of 70.0%. Conclusions: The preliminary data suggested a potential application of MALDI-TOF-MS combined with magnetic beads. The model comprising 5 promising biomarkers can differentiate individuals with PD and the healthy subjects precisely. Copyright (C) 2011 S. Karger AG, Basel
基金：
the National High Technology Research and Development Program (‘863’ Program) of China (2006AA02A408), the National Basic Research Program (‘973’ Program) of China (2011CB504101),
the National Natural Science Foundation of China (30271437, 30270482, 30430280, 81071014), the Natural Science Foundation of Beijing (7102076, 7022011)
and the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under Jurisdiction of Beijing Municipality (PHR200907113).
第一作者机构：[1]Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing , China[*]Beijing Institute of Geriatrics Xuanwu Hospital of Capital University of Medical Sciences 45# Changchun Street, Beijing 100053 (China)
通讯作者：
通讯机构：[*]Beijing Institute of Geriatrics Xuanwu Hospital of Capital University of Medical Sciences 45# Changchun Street, Beijing 100053 (China)
推荐引用方式(GB/T 7714)：
Yao-Hua Li,Jian Wang,Xiao-Li Zheng,et al.Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Combined with Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease[J].EUROPEAN NEUROLOGY.2011,65(2):105-111.doi:10.1159/000323427.
APA：
Yao-Hua Li,Jian Wang,Xiao-Li Zheng,Yan-Li Zhang,Xin Li...&Piu Chan.(2011).Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Combined with Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease.EUROPEAN NEUROLOGY,65,(2)
MLA：
Yao-Hua Li,et al."Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Combined with Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease".EUROPEAN NEUROLOGY 65..2(2011):105-111
