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Increased expression of urotensin II and GPR14 in patients with cirrhosis and portal hypertension

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机构: [1]Department of General Surgery, Xuan Wu Hospital, Capital Medical University, Beijing [2]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University,Beijing [3]Liver Research Center, Beijing Friendship Hospital, Capital Medical University,Beijing [4]Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P.R. China
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关键词: liver cirrhosis portal hypertension urotensin II GPR14

摘要:
Urotensin II (UII) and its receptor (UT or GPR14) are involved in liver fibrosis and portal hypertension. Nevertheless, expression of the UII/UT system in the liver of patients with portal hypertension has not been elucidated. UII and UT gene expression were quantified in liver biopsy samples from patients with hepatitis-B-virus-associated cirrhosis and portal hypertension, and from normal controls by using quantitative real-time PCR. The liver distribution of UT was determined by means of immunohistochemistry and immunofluorescence. Western blot analysis was used to assess liver levels of UT. Simultaneously, we measured intra-operative free portal venous pressure (FPVP) and collected plasma for UII measurement by ELISA. UT expression at the mRNA and protein level was enhanced significantly in the liver of patients with cirrhosis and portal hypertension, compared with that in healthy controls. UT protein expression was concentrated mainly in the Kupffer cells and sinusoidal endothelial cells. In cirrhotic tissue, UII gene expression was increased 5-fold in comparison to that in normal liver tissue. Plasma UII level was higher in cirrhotic patients compared with controls and was correlated with FPVP (r=0.807; P<0.001) and UII mRNA in the liver (r=0.802; P<0.001). These findings suggest that the intrahepatic UII/UT system has an important pathophysiological role in cirrhosis and portal hypertension.

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出版当年[2009]版:
大类 | 4 区 医学
小类 | 4 区 医学:研究与实验
最新[2023]版:
大类 | 3 区 医学
小类 | 3 区 医学:研究与实验
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出版当年[2008]版:
Q3 MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版:
Q1 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子: 最新[2023版] 最新五年平均 出版当年[2008版] 出版当年五年平均 出版前一年[2007版] 出版后一年[2009版]

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第一作者机构: [1]Department of General Surgery, Xuan Wu Hospital, Capital Medical University, Beijing [2]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University,Beijing
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通讯机构: [*1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An Road, Beijing 100050, P.R. China
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