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Ginsenoside Rg1 attenuates beta-amyloid-induced apoptosis in mutant PS1 M146L cells

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收录情况： ◇ SCIE

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机构： [a]Department of Neurology, Xuan Wu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, China [b]Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences and Peking Union Medical College, China [c]Beijing Institute of Geriatrics, Xuan Wu Hospital, Capital Medical University, China

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关键词： ginsenoside Rg1 Alzheimer's disease apoptosis beta-amyloid caspase-3

摘要：

Ginsenoside Rg1 (Rg1) is a pharmaceutically active component of ginseng, and is neuroprotective as reported. This experiment investigated whether Rg1 is effective on injury or apoptosis of Chinese hamster ovary (CHO) cells as induced by A beta(25-35), or by excessive A beta(1-42) and the mechanism involved. We used different Rg1 doses to pretreat CHO cells stably expressing APP751 and either wild-type PS1 (WT) or mutant PS1 (M146L) for 24 h. Cell viability and apoptosis were examined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay, terminal deoxynucleotidyl-transferase-mediated dUTP transferase nick-end labeling (TUNEL), and fluorescent annexin V/propidium iodide (Annexin V-FITC/PI) staining. The expression of A beta(1-42) and caspase-3 was investigated with immunofluorescent staining. Our results reveal that pretreatment with 25 mu M Rg1 can improve viability in cells injured by A beta(25-35), inhibit the intracellular A beta(1-42)-induced apoptosis in mutant PSI M146L cells, and reduce the levels of A beta(1-42) and active caspase-3. This study demonstrated that Rg1 can reduce the production of A beta(1-42) and the activation of caspase-3, as a result, to attenuate the cell apoptosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

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出版当年[2007]版：

大类 | 3 区医学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区神经科学

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出版当年[2006]版：

Q3 NEUROSCIENCES

最新[2023]版：

Q3 NEUROSCIENCES

影响因子： 2.5 最新[2023版] 2.5 最新五年平均 2.092 出版当年[2006版] 0 出版当年五年平均 1.898 出版前一年[2005版] 2.085 出版后一年[2007版]

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第一作者机构： [a]Department of Neurology, Xuan Wu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, China

通讯作者：

通讯机构： [a]Department of Neurology, Xuan Wu Hospital, Capital Medical University, 45 Changchun Street, Beijing 100053, China

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