机构：[a]Institute of Cerebrovascular Diseases, Xuan Wu Hospital, Capital Medical University, Beijing, 100053, China首都医科大学宣武医院[b]Department of Biological Science and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China[c]Department of Neurobiology, Capital Medical University, Beijing, 100054, China
Insulin-like growth factor 1 (IGF-1) has important functions in the brain, including metabolic, neurotrophic, neuromodulatory and neuroendocrine actions, and it also prevents beta amyloid-induced death of hippocampal neurons. However, its functions in the synaptic excitability remain uncertain. Here we investigated the effects of IGF-1 on synaptic excitability in cultured rat hippocampal neurons using whole-cell patch clamp recordings. Incubation the hippocampal neurons with different concentrations of IGF-1 for 24 h or 30 min significantly increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs), but had no effect on the frequency of miniature EPSCs (mEPSCs) and spontaneous inhibitory postsynaptic currents (sIPSCs). The mean amplitudes, rise, and decay kinetics of sEPSCs, mEPSCs, and sIPSCs were not significantly affected by IGF-1, indicating that IGF-1 increased the probability of neurotransmitter release but did not modulate postsynaptic receptors. The effects of IGF-1 were mediated by mitogen-activated protein kinase (MAPK). IGF-1 activated the ERK1/2 signaling pathway in cultured hippocampal neurons, and the inhibitor PD98059 blocked the enhancement of sEPSCs induced by IGF-1. These results demonstrated the regulatory function of IGF-1 on synaptic excitability in hippocampal neurons and its underlying signaling mechanism. (C) 2007 Elsevier Inc. All rights reserved.
第一作者机构：[a]Institute of Cerebrovascular Diseases, Xuan Wu Hospital, Capital Medical University, Beijing, 100053, China[b]Department of Biological Science and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China
通讯作者：
通讯机构：[a]Institute of Cerebrovascular Diseases, Xuan Wu Hospital, Capital Medical University, Beijing, 100053, China[b]Department of Biological Science and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China
推荐引用方式(GB/T 7714)：
Changhong Xing,Yanling Yin,Rui Chang,et al.Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons[J].EXPERIMENTAL NEUROLOGY.2007,205(1):222-229.doi:10.1016/j.expneurol.2007.01.029.
APA：
Changhong Xing,Yanling Yin,Rui Chang,Xiaoming Gong,Xiangping He&Zuoping Xie.(2007).Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons.EXPERIMENTAL NEUROLOGY,205,(1)
MLA：
Changhong Xing,et al."Effects of insulin-like growth factor 1 on synaptic excitability in cultured rat hippocampal neurons".EXPERIMENTAL NEUROLOGY 205..1(2007):222-229
