机构：[a]Institute of Cerebrovascular Diseases, Xuanwu Hospital, The Capital University of Medical Sciences, Beijing 100053, China首都医科大学宣武医院[b]Department of Biological Science and Technology, Tsinghua University, Beijing 100084, China[c]Department of Neurobiology, The Capital University of Medical Sciences, Beijing 100054, China
insulin-like growth factor 1 (IGF-1) has important functions in the brain, including metabolic, neurotrophic, neuromodulatory, and neuroendocrine actions, and it is also, prevents amyloid beta-induced death of hippocampal neurons. However, its functions on the voltage-gated ion channels in hippocampus remain uncertain. In the present study, we investigated the effects of IGF-1 on voltage-gated potassium, sodium, and calcium channels in the cultured rat hippocampal neurons using the whole-cell patch clamp recordings. Following incubation with different doses of IGF-1 for 24 h, a block of the peak transient A-type K+ currents amplitude (IC50): 4.425 ng/ml, Hill coefficient: 0.621) was observed. In addition, after the application of IGF-1, the amplitude of high-voltage activated Ca2+ currents significantly increased but activation kinetics did not significantly alter (V-1/2: -33.45 +/- 1.32 mV, k = 6.16 +/- 1.05) compared to control conditions (V-1/2: -33.19 +/- 2.28 mV, k = 7.26 +/- 1.71). However, the amplitude of Na+, K+, and low-voltage activated Ca2+ currents was not affected by the application of IGF-1. These data suggest that IGF-1 inhibits transient A-type K+ currents and enhances high-voltage-activated Ca2+ currents, but has no effects on Na+ and low-voltage-activated Ca2+ currents. (c) 2005 Elsevier B.V. All rights reserved.
第一作者机构：[a]Institute of Cerebrovascular Diseases, Xuanwu Hospital, The Capital University of Medical Sciences, Beijing 100053, China
通讯作者：
通讯机构：[a]Institute of Cerebrovascular Diseases, Xuanwu Hospital, The Capital University of Medical Sciences, Beijing 100053, China[b]Department of Biological Science and Technology, Tsinghua University, Beijing 100084, China
推荐引用方式(GB/T 7714)：
Changhong Xing,Yanling Yin,Xiangping He,et al.Effects of insulin-like growth factor 1 on voltage-gated ion channels in cultured rat hippocampal neurons[J].BRAIN RESEARCH.2006,1072(1):30-35.doi:10.1016/j.brainres.2005.10.091.
APA：
Changhong Xing,Yanling Yin,Xiangping He&Zuoping Xie.(2006).Effects of insulin-like growth factor 1 on voltage-gated ion channels in cultured rat hippocampal neurons.BRAIN RESEARCH,1072,(1)
MLA：
Changhong Xing,et al."Effects of insulin-like growth factor 1 on voltage-gated ion channels in cultured rat hippocampal neurons".BRAIN RESEARCH 1072..1(2006):30-35
