机构：[1]Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, Changchun, Jilin 130021, China.[2]Department of Orthopaedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130031, China[3]Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China老年医学科首都医科大学宣武医院
Objective To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, EL-1beta, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032 +/- 2.489%,41.580 +/- 5.101% and 34.958 +/- 5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1beta, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application.
基金：
the National Natural Science Foundation of China (No.39970741)
a grant from the the Science and Technology Foundation of Jilin Health Administration (No. 200131)
a grant from the Youth Teacher Foundation of Jilin University (No. 2003QN013)
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2004]版：
无
最新[2023]版：
大类|3 区医学
小类|4 区环境科学4 区公共卫生、环境卫生与职业卫生
JCR分区：
出版当年[2003]版：
Q3ENVIRONMENTAL SCIENCESQ4PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
第一作者机构：[1]Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, Changchun, Jilin 130021, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, Changchun, Jilin 130021, China.
推荐引用方式(GB/T 7714)：
LI GUO,FEI YIN,HONG-QI MENG,et al.Differentiation of mesenchymal stem cells into dopaminergic neuron-like cells in vitro[J].BIOMEDICAL AND ENVIRONMENTAL SCIENCES.2005,18(1):36-42.
APA：
LI GUO,FEI YIN,HONG-QI MENG,LING LING,TA-NA HU-HE...&HONG-XUE FAN.(2005).Differentiation of mesenchymal stem cells into dopaminergic neuron-like cells in vitro.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,18,(1)
MLA：
LI GUO,et al."Differentiation of mesenchymal stem cells into dopaminergic neuron-like cells in vitro".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 18..1(2005):36-42
