详情页 - 首都医科大学宣武医院知识库

当前位置：首页 > 详情页

Assays to examine endothelial cell migration, tube formation, and gene expression profiles

文献详情

资源类型：

作者：

机构： [1]Neuroprotection Research Laboratory, Department of Radiology and Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, USA [2]Department of Pediatrics Harvard Medical School, Massachusetts General Hospital, Boston, USA [3]Cerebrovascular Research Center, Xuanwu Hospital Capital Medical University, Beijing, China

出处：

DOI：

ISSN：

关键词： Angiogenesis Endothelial cells Matrigel matrix Microparticles Migration Transcriptome Tube formation

摘要：

Common methods for studying angiogenesis in vitro include the tube formation assay, the migration assay, and the study of the endothelial genome. The formation of capillary-like tubes in vitro on basement membrane matrix mimics many steps of the angiogenesis process in vivo and is used widely as a screening test for angiogenic or antiangiogenic factors. Other assays related to the study of angiogenesis include the cell migration assay, the study of gene expression changes during the process of angiogenesis, and the study of endothelial-derived microparticles. Protocols for these procedures will be described here. © 2014 Springer Science+Business Media New York.

语种：

第一作者：

第一作者机构： [1]Neuroprotection Research Laboratory, Department of Radiology and Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, USA

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles [2]Lipocalin-2 enhances angiogenesis in rat brain endothelial cells via reactive oxygen species and iron-dependent mechanisms [3]LncRNA WTAPP1 Promotes Migration and Angiogenesis of Endothelial Progenitor Cells via MMP1 Through MicroRNA 3120 and Akt/PI3K/Autophagy Pathways [4]Platelet-Derived Exosomes Affect the Proliferation and Migration of Human Umbilical Vein Endothelial Cells via miR-126 [5]Exosomes secreted from osteocalcin-overexpressed endothelial progenitor cells promoted endothelial cell angiogenesis. [6]Effects of Hypoxia on Cerebral Microvascular Angiogenesis: Benefits or Damages? [7]ER stress dependent microparticles derived from smooth muscle cells promote endothelial dysfunction during thoracic aortic aneurysm and dissection [8]Wnt3a promoted the therapeutic of angiogenesis on lower leg ischemia with endothelial progenitor cells [9]Overexpression of osteopontin induces angiogenesis of endothelial progenitor cells via the av beta 3/PI3K/AKT/eNOS/NO signaling pathway in glioma cells [10]Effect of different enamel matrix derivative proteins on behavior and differentiation of endothelial cells