机构：[1]Department of Interventional Radiology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000[2]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China
MicroRNAs (miRNAs) are considered to be critical mediators of gene expression with respect to tumor progression, although their role in ischemia?induced angiogenesis is poorly characterized, including in peripheral arterial disease (PAD). Furthermore, the underlying mechanism of action of specific miRNAs in PAD remains unknown. Reverse transcription?quantitative polymerase chain reaction analysis revealed that microRNA?93 (miR?93) was significantly upregulated in patients with PAD and in the EA.hy926 endothelial cells in response to hypoxia. Additionally, miRNA (miR)?93 promoted angiogenesis by enhancing proliferation, migration and tube formation. Cyclin dependent kinase inhibitor?1A (CDKN1A), verified as a potential target gene of miR?93, was inhibited by overexpressed miR?93 at the protein and mRNA expression levels. Furthermore, a hind?limb ischemia model served to evaluate the role of miR?93 in angiogenesis in?vivo, and the results demonstrated that miR?93 overexpression enhanced capillary density and perfusion recovery from hind?limb ischemia. Taken together, miR?93 was indicated to be a promising target for pharmacological regulation to promote angiogenesis, and the miR?93/CDKN1A pathway may function as a novel therapeutic approach in PAD.
基金：
The present study was supported by grants from the National
Natural Science Foundation of China (grant no. 81770483),
the Jiangsu Provincial Science and Technology Office (grant
no. BL2014043), and the Suzhou Health and Family Planning
Commission Program (grant no. LC ZX201504).
第一作者机构：[1]Department of Interventional Radiology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000[2]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215000, P.R. China
推荐引用方式(GB/T 7714)：
Shu Xiaojun,Mao Youjun,Li Zhengfei,et al.MicroRNA?93 regulates angiogenesis in peripheral arterial disease by targeting CDKN1A.[J].Molecular medicine reports.2019,19(6):5195-5202.doi:10.3892/mmr.2019.10196.
APA：
Shu Xiaojun,Mao Youjun,Li Zhengfei,Wang Wenhui,Chang Yaowen...&Li Xiao-Qiang.(2019).MicroRNA?93 regulates angiogenesis in peripheral arterial disease by targeting CDKN1A..Molecular medicine reports,19,(6)
MLA：
Shu Xiaojun,et al."MicroRNA?93 regulates angiogenesis in peripheral arterial disease by targeting CDKN1A.".Molecular medicine reports 19..6(2019):5195-5202
