机构：[1]Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China[2]Department of Head and Neck Surgery, Affiliated Tumor Hospital of Nantong University, Nantong Tumor Hospital, Nantong, Jiangsu, China[3]Departments of Otorhinolaryngology,Affiliated Hospital of Nantong University, Nantong, Jiangsu, China[4]Departments of Clinical Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China
Melanoma-associated antigen (MAGE) family genes are reported to play important roles in the development of human cancers. However, the relationship between the expression of MAGE-A9 and clinicopathological characteristics in human laryngeal carcinoma remains unclear. This study aimed to examine the expression of MAGE-A9, and to evaluate the clinical significance of its expression in human laryngeal squamous cell carcinoma (LSCC).
Quantitative real-time reverse transcription-PCR (qPCR) and immunohistochemistry (IHC) were performed to characterize the expression of MAGE-A9 in LSCC tissues and tumor-adjacent normal tissues. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of patients with LSCC.
The expression of MAGE-A9 was significantly higher in LSCC than in tumor-adjacent normal tissues. Cytoplasmic expression of MAGE-A9 was detected in 70 of 123 (56.9%) LSCC specimens. Levels of MAGE-A9 in LSCC were related to histopathological grade (P = 0.024). Kaplan-Meier survival and Cox regression analysis revealed that MAGE-A9 expression level and lymph node metastasis were independent prognostic factors of LSCC (P = 0.005; P = 0.001, respectively).
Our study suggests that MAGE-A9 expression is a prognostic biomarker for LSCC patients. High expression of MAGE-A9 indicates unfavorable survival outcome in LSCC patients.
基金：
This study was supported by a grant from the Jiangsu Natural Science Fund in 2014 and Jiangsu Province’s Key Medical Department in 2011.
第一作者机构：[1]Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China[2]Department of Head and Neck Surgery, Affiliated Tumor Hospital of Nantong University, Nantong Tumor Hospital, Nantong, Jiangsu, China
通讯作者：
通讯机构：[*1]Department of Radiotherapy & Oncology Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Liang Han,Bin Jiang,Hao Wu,et al.Expression and prognostic value of MAGE-A9 in laryngeal squamous cell carcinoma.[J].International journal of clinical and experimental pathology.2014,7(10):6734-42.
APA：
Liang Han,Bin Jiang,Hao Wu,Shu Zhang&Xueguan Lu.(2014).Expression and prognostic value of MAGE-A9 in laryngeal squamous cell carcinoma..International journal of clinical and experimental pathology,7,(10)
MLA：
Liang Han,et al."Expression and prognostic value of MAGE-A9 in laryngeal squamous cell carcinoma.".International journal of clinical and experimental pathology 7..10(2014):6734-42
