机构：[a]Otolaryngology Head and Neck Surgery, Hainan Branch of Chinese PLA General Hospital, Sanya, China[b]Otolaryngology Head and Neck Surgery, General Hospital of People’s Liberation Army, Beijing, China[c]Peking Union Medical College, Department of Head and Neck Surgery, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing, China[d]Otolaryngology, Capital Medical University Affiliated Anzhen Hospital, Beijing, China临床科室耳鼻咽喉头颈外科首都医科大学附属安贞医院[e]Otolaryngology Head and Neck Surgery, Weifang Medical University, Weifang, China[f]Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China[g]Otolaryngology Head and Neck Surgery, Hainan Branch of Chinese PLA General Hospital, Sanya, China
Background: Until now, little is known about the role of miRNAs in the invasion and metastasis of Laryngeal squamous cell carcinoma (LSCC). Objectives: This study aimed to explore the relationship between microRNA and the invasion and metastasis of LSCC. Material and methods: The highly metastatic laryngocarcinoma cells were obtained from the established animal model with spontaneous lymph node metastasis of LSCC in our previous study. MicroRNA expression profiling and bioinformatic analysis were performed to analyze the microRNA expression changes in the highly metastatic laryngocarcinoma cells and the parental tumor cells (HEP-2). RT-PCR was performed for further validation of the result of microarray. Results: A total of 40 microRNAs were found to be significantly altered in the highly metastatic laryngocarcinoma cells compared to controls. Bioinformatic analysis identified that 19 key microRNAs might involve in LSCC development. Moreover, RT-PCR confirmed that miR-25, miR-100, miR-125b-5p and let-7g were differentially expressed in different laryngocarcinoma cells and human tumor specimens. Conclusions and significance: Our findings suggest that microRNA play an important role in the invasion and metastasis of LSCC, and provide the clues for studying the function of microRNA as well as opportunities to analyze the complex molecular abnormalities driving LSCC progression.
基金：
This project was supported by Hainan Provincial Natural Science
Foundation of China (No. 818MS154) and Primary Research &
Developement Plan of Hainan Province (No. ZDYF2017076).
第一作者机构：[a]Otolaryngology Head and Neck Surgery, Hainan Branch of Chinese PLA General Hospital, Sanya, China[b]Otolaryngology Head and Neck Surgery, General Hospital of People’s Liberation Army, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[f]Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, China[g]Otolaryngology Head and Neck Surgery, Hainan Branch of Chinese PLA General Hospital, Sanya, China[*1]Hainan Branch of Chinese PLA General Hospital, Otolaryngology Head and Neck Surgery, Haitang Bay, Sanya, Sanya 572013, China
推荐引用方式(GB/T 7714)：
Liwei Chen,Shaoyan Liu,Kun Li,et al.Evaluation of microRNA expression profiling in highly metastatic laryngocarcinoma cells[J].ACTA OTO-LARYNGOLOGICA.2018,138(12):1105-1111.doi:10.1080/00016489.2018.1508887.
APA：
Liwei Chen,Shaoyan Liu,Kun Li,Jixia Qi,Chenqing Liu...&Mingbo Liu.(2018).Evaluation of microRNA expression profiling in highly metastatic laryngocarcinoma cells.ACTA OTO-LARYNGOLOGICA,138,(12)
MLA：
Liwei Chen,et al."Evaluation of microRNA expression profiling in highly metastatic laryngocarcinoma cells".ACTA OTO-LARYNGOLOGICA 138..12(2018):1105-1111
