机构：[1]Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China[2]Medprin Regenerative Medical Technologies Co., Ltd, Shenzhen 518102, China[3]The Second Affiliated Hospital of Soochow University, Suzhou 215004, China苏州大学附属第二医院[4]Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属天坛医院
Currently, there is still a lack of appropriate in vitro model for studying lung cancers, especially for recapitulating their invasion and metastasis properties. To develop an appropriate in vitro model for lung cancer research, low-temperature molding principle of biological manufacturing and 3D bioprinting was used in this study to fabricate a cell-laden hydrogel grid scaffold structure, using gelatin-sodium alginate-lung cancer cell A549/95-D suspension as the bio-ink. Cells distributed evenly in this model with high viability, and can be cultured sustainably. This model can be cultured for up to 28 days and maintained its structural integrity. Histology, gene analysis, and scratch test showed that 3D printed cells had enhanced invasion and migration capability compared to those cultured in 2D environment, indicating that the in vitro model developed in this study was more biomimetic compared to 2D models, and it is highly valuable in biomedical research.
基金：
This project is funded by the Shenzhen Special Fund
for Global Experts Team, China (no. KQTD201209) and the Key
Research and Development Projects of People’s Liberation Army (no.
BWS17J036).
第一作者机构：[1]Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
通讯作者：
通讯机构：[1]Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
推荐引用方式(GB/T 7714)：
Xiong Wang,Xinzhi Zhang,Xingliang Dai,et al.Tumor-like lung cancer model based on 3D bioprinting[J].3 BIOTECH.2018,8(12):-.doi:10.1007/s13205-018-1519-1.
APA：
Xiong Wang,Xinzhi Zhang,Xingliang Dai,Xuanzhi Wang,Xinda Li...&Tao Xu.(2018).Tumor-like lung cancer model based on 3D bioprinting.3 BIOTECH,8,(12)
MLA：
Xiong Wang,et al."Tumor-like lung cancer model based on 3D bioprinting".3 BIOTECH 8..12(2018):-
