机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China[2]Medprin Biotech GmbH, Gutleutstra?e 163-167, Frankfurt am Main, D-60327, Germany[3]Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People’s Republic of China[4]East China Institute of Digital Medical Engineering, Shangrao, 334000, China[5]Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055, People’s Republic of China
Glioma stem cells (GSCs) are thought to be the root cause of tumor recurrence and drug resistance in glioma patients. In-depth study of GSCs is of great significance for developing the treatment strategies of glioma. Unfortunately, it is difficult and takes complicated process to obtain GSCs. Therefore, establishing an ideal in vitro model for enriching GSCs will greatly promote the study of GSCs. In this study, the stemness properties of glioma cells were enhanced in three-dimensional (3D) bioprinted tumor model. Furthermore, the possible molecular mechanism of GSCs enrichment: epithelial-mesenchymal transition (EMT) was explored. Compared with two-dimensional cultured cells, the proportion of GSCs and EMT-related genes in 3D cultured cells were significantly increased. Moreover, the 3D cultured glioma cells with improved stemness properties resulted in higher drug resistance in vitro and tumorigenicity in vivo. Taken together, 3D bioprinted glioma cell-laden scaffold provides a proper platform for the enrichment of GSCs and it is expected to further promote the research on glioma drug resistance. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 383-391, 2019.
基金：
This study was financially supported by the following programs:
Clinical Cutting-edge Technology, Social Development
Projects in Jiangsu Province (BE2016668); Guangdong Innovative
Research Team Program, China (No. 2011S055); The
Shenzhen Special Fund for Global Experts Team, China
(No. KQTD201209); Postgraduate Research & Practice Innovation
Program of Jiangsu Province (KYCX17_1993); The National
Natural Science Foundation of China (grant nos. 81702457);Suzhou Science and Technology Project (SYS201723); China
National Nuclear Corporation Youth Innovation TeamProject.
第一作者机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, People’s Republic of China[3]Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People’s Republic of China[5]Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055, People’s Republic of China
推荐引用方式(GB/T 7714)：
Xuanzhi Wang ,Xingliang Dai ,Xinzhi Zhang ,et al.3D bioprinted glioma cell-laden scaffolds enriching glioma stem cells via epithelial-mesenchymal transition[J].JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A.2019,107(2):383-391.doi:10.1002/jbm.a.36549.
APA：
Xuanzhi Wang,,Xingliang Dai,,Xinzhi Zhang,,Cheng Ma,,Xinda Li,...&Qing Lan.(2019).3D bioprinted glioma cell-laden scaffolds enriching glioma stem cells via epithelial-mesenchymal transition.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,107,(2)
MLA：
Xuanzhi Wang,,et al."3D bioprinted glioma cell-laden scaffolds enriching glioma stem cells via epithelial-mesenchymal transition".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 107..2(2019):383-391
