机构：[1]Department of Cardiovascular Surgery II, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China临床科室职能科室临床流行病与循证医学中心心脏外科首都医科大学附属北京儿童医院
OBJECTIVE: Myocardial cell apoptosis is an important pathologic basis of ischemia-reperfusion injury (I/R). PI3K/Akt signaling pathway involves in cell growth. survival, and apoptosis regulation, thus playing an important role in the protection of I/R injury. PTEN is a negative regulatory factor of PI3K/Akt signaling pathway. This study established rat I/R injury model after AMI and myocardial cell I/R injury model to explore the regulatory role of PTEN-PI3K/Akt signaling pathway in myocardial I/R injury in vivo and in vitro. MATERIALS AND METHODS: Rat myocardial I/R injury model was established. PTEN and p-Akt expressions in myocardial tissue were compared. H9C2 cells were incubated in I/R condition for 12 h, followed by reoxygenation for 12 h. H9C2 cells were divided into three groups, including I/R+pSicoR-Blank, I/R+pSicoR-PTEN, and I/R+pSicoR-PTEN+VO-Ohpic. PTEN, p-Akt, Bcl-2. and Bax expressions were detected. Cell apoptosis was measured by flow cytometry. RESULTS: PTEN expression significantly increased, while p-Akt level markedly declined in myocardial tissue in I/R group compared with Sham group. Temporary PTEN downregulation and p-Akt elevation appeared at 2 h after I/R. I/R treatment markedly enhanced PTEN and Bax expressions, increased cell apoptosis, and reduced p-Akt and BcI-2 levels. PTEN overexpression significantly enhanced Bax expression and cell apoptosis, while declined p-Akt and Bch-2 in H9C2 after I/R. PTEN inhibited by VO-Ohpic markedly downregulated p-Akt and Bcl-2 expressions, whereas reduced Bax level and cell apoptosis. CONCLUSIONS: The overexpression of PTEN aggravated myocardial cell apoptosis after I/R. The blockage of PTEN enhanced PI3K/Akt signaling pathway and attenuated cell apoptosis induced by I/R.
基金：
Special Fund of The Pediatric Medical Coordinated Development Center of Beijing Municipal Administration [XTYB201819]; Research on the application of clinical characteristics of the Beijing Municipal Science and Technology Commission [Z171100001017048]; Beijing Municipal Education Commission Science and Technology Plan General Project [KM201910025010]; Beijing Children's Hospital; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [GPY201814]
第一作者机构：[1]Department of Cardiovascular Surgery II, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China
通讯作者：
通讯机构：[1]Department of Cardiovascular Surgery II, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China
推荐引用方式(GB/T 7714)：
Zhu Y-B,Ding N.,Yi H-L,et al.The expression of overexpressed PTEN enhanced IR-induced apoptosis of myocardial cells[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2019,23(10):4406-4413.doi:10.26355/eurrev_201905_17948.
APA：
Zhu, Y-B,Ding, N.,Yi, H-L&Li, Z-Q.(2019).The expression of overexpressed PTEN enhanced IR-induced apoptosis of myocardial cells.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,23,(10)
MLA：
Zhu, Y-B,et al."The expression of overexpressed PTEN enhanced IR-induced apoptosis of myocardial cells".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 23..10(2019):4406-4413
