机构：[1]Division of Cardiology, Anzhen Hospital Capital Medical University, Beijing, China临床科室心脏内科中心首都医科大学附属安贞医院[2]Department of Pharmacy, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[3]Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, China首都医科大学附属安贞医院[4]Department of Internal Medicine, Icahn School of Medicine at Mount Sinai, New York, New York[5]Department of Echocardiography, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China医技科室职能科室临床流行病与循证医学中心心脏彩超首都医科大学附属北京儿童医院
ObjectiveThis study was designed to explore the protective effect of 2,3,5,4-tetrahydroxystilbene-2-O--d-glucoside (TSG) against ischemia/reperfusion (I/R) injury-induced cardiomyocytes apoptosis. MethodsThe H9c2 cell I/R injury model was induced by simultaneous shortage of nutrients and oxygen. TSG administration (0.10, 0.25, and 0.50mM) was performed before and during I/R stimulation. Cell apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Expression of cell-related proteins was detected to assess the effect of TSG on cell apoptosis. ResultsI/R injury induced significant cell apoptosis. Significantly decreased Bcl-2 and increased Bax, caspase-3, and p-Akt expression (P<0.01) was detected in the cell model of I/R injury. In contrast, TSG administration eliminated all the changes induced by I/R injury in a dose-dependent manner. Compared with the H9c2 cell model of I/R injury, the H9c2 cells treated with 0.50mM TSG showed the lowest cell apoptosis percentage, the highest expression of Bcl-2, and the lowest expression of Bax, caspase-3, and p-Akt (P<0.01). ConclusionWe confirmed that the protective effect of TSG against I/R injury-induced cell apoptosis in H9c2 in vitro was associated with the Bcl-2/Bax ratio, caspase-3, and Akt activation.
基金：
Beijing Lab for Cardiovascular Precision Medicine [PXM2016-014226-000023]
第一作者机构：[1]Division of Cardiology, Anzhen Hospital Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[5]Department of Echocardiography, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China[*1]Department of Echocardiography, Beijing Children’s Hospital, Capital Medical University, National Center for Childre’s Health, Xicheng District, Nanlishi Road 56, Beijing, China.
推荐引用方式(GB/T 7714)：
Sun Tao,Liu Han,Cheng Yutong,et al.2,3,5,4 '-Tetrahydroxystilbene-2-O-beta-d-glucoside eliminates ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis involving in Bcl-2, Bax, caspase-3, and Akt activation[J].JOURNAL OF CELLULAR BIOCHEMISTRY.2019,120(7):10972-10977.doi:10.1002/jcb.27949.
APA：
Sun, Tao,Liu, Han,Cheng, Yutong,Yan, Lixiao,Krittanawong, Chayakrit...&Zhang, Hongju.(2019).2,3,5,4 '-Tetrahydroxystilbene-2-O-beta-d-glucoside eliminates ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis involving in Bcl-2, Bax, caspase-3, and Akt activation.JOURNAL OF CELLULAR BIOCHEMISTRY,120,(7)
MLA：
Sun, Tao,et al."2,3,5,4 '-Tetrahydroxystilbene-2-O-beta-d-glucoside eliminates ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis involving in Bcl-2, Bax, caspase-3, and Akt activation".JOURNAL OF CELLULAR BIOCHEMISTRY 120..7(2019):10972-10977
