机构：[1]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China[2]Institute of Neuroscience, Soochow University, Suzhou 215000, China[3]Department of Anesthesiology, Stony Brook University School of Medicine, Stony Brook, NY 11794, USA
Aim: To investigate the role of extracellular signal-regulated kinases (ERKs) in sevoflurane post-conditioning induced cardioprotection in vitro. Methods: Isolated rat hearts were subjected to 30 min ischemia followed by 120 min reperfusion (I/R). Sevoflurane post-conditioning was carried out by administration of O-2-enriched gas mixture with 3% sevoflurane (SEVO) for 15 min from the onset of reperfusion. Cardiac functions, myocardial infarct size, myocardial ATP and NAD(+) contents, mitochondrial ultrastructure, and anti-apototic and anti-oncosis protein levels were measured. Results: Sevoflurane post-conditioning significantly improved the heart function, decreased infarct size and mitochondria damage, and increased myocardial ATP and NAD(+) content in the I/R hearts. Furthermore, sevoflurane post-conditioning significantly increased the levels of p-ERK and p-p70S6K, decreased the levels of porimin, caspase-8, cleaved caspase-3, and cytosolic cytochrome c in the I/R hearts. Co-administration of the ERK1/2 inhibitor PD98059 (20 mu mol/L) abolished the sevoflurane-induced protective effects against myocardial I/R. Conclusion: Sevoflurane post-conditioning protects isolated rat hearts against myocardial I/R injury and inhibits cell oncosis and apoptosis via activation of the ERK1/2 pathway.
基金：
This work is supported by the National Natural Science Foundation of China (Grant No 81372024 ), the
Technology Bureau of Suzhou, China (Grants No SYS201130, SYSD2012085, and KJXW2011015 ; SYS201125 and SYS201340 ).
第一作者机构：[1]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China[2]Institute of Neuroscience, Soochow University, Suzhou 215000, China
推荐引用方式(GB/T 7714)：
Hong XIE,Jing ZHANG,Jiang ZHU,et al.Sevoflurane post-conditioning protects isolated rat hearts against ischemia-reperfusion injury via activation of the ERK1/2 pathway[J].ACTA PHARMACOLOGICA SINICA.2014,35(12):1504-13.doi:10.1038/aps.2014.78.
APA：
Hong XIE,Jing ZHANG,Jiang ZHU,Li-xin LIU,Mario REBECCHI...&Chen WANG.(2014).Sevoflurane post-conditioning protects isolated rat hearts against ischemia-reperfusion injury via activation of the ERK1/2 pathway.ACTA PHARMACOLOGICA SINICA,35,(12)
MLA：
Hong XIE,et al."Sevoflurane post-conditioning protects isolated rat hearts against ischemia-reperfusion injury via activation of the ERK1/2 pathway".ACTA PHARMACOLOGICA SINICA 35..12(2014):1504-13
医学