机构：[1]Department of Laboratory Medicine, Shanghai Sixth People's Hospital Jinshan Branch, Shanghai, China[2]Fangsong Community Health Centre, Shanghai, China[3]Department of Pulmonary Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China首都医科大学附属天坛医院[4]Department of Pathology, Shanghai Sixth People's Hospital Jinshan Branch, Shanghai, China[5]Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, Shanghai, China
Long noncoding RNA KCNQ1OT1 participates in the regulation of imprinted genes within the kcnq1 domain. But its roles in carcinogenesis and metastasis remain largely elusive. Herein, we evaluated its potential in non-small-cell lung cancer (NSCLC) progression. We demonstrated that the KCNQ1OT1 level was upregulated in NSCLC tissues and cell lines. High KCNQ1OT1 level correlated with poor overall and progression-free survival in NSCLC patients. KCNQ1OT1 facilitated proliferation, migration, and invasion in H460 cells. Furthermore, knockdown of KCNQ1OT1 reduced the expression of HSP90AA1. KCNQ1OT1 presented a positive correlation with HSP90AA1 which predicted the tumor progression in NSCLC from The Cancer Genome Atlas database. Intriguingly, KCNQ1OT1 modulated HSP90AA1 expression by sponging miR-27b-3p. MiR-27b-3p counteracted the effect of KCNQ1OT1 on HSP90AA1 expression, H460 cell migration, and invasion. These data revealed a role for KCNQ1OT1 as an oncogene through miR-27b-3p/HSP90AA1 axis during NSCLC progression.
基金：
Shanghai Municipal Commission of Health and Family Planning research project [201640131]; Shanghai Sixth People's Hospital Medical Group Foundation [2015-01]; Science and Technology Committee of Shanghai Jinshan District Innovation Foundation [2015-3-17]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81572692]
