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Intracerebral Hemorrhage–Induced Brain Injury in Rats: the Role of Extracellular Peroxiredoxin 2

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机构： [1]Department of Neurosurgery, University ofMichigan, R5018 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA [2]Department of Neurology, Beijing Tiantan Hospital, CapitalMedical University, Beijing, China

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关键词： Blood-brain barrier Brain swelling Intracerebral hemorrhage Neutrophils Peroxiredoxin 2

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Red blood cell (RBC) lysis within the hematoma causes brain injury following intracerebral hemorrhage. Peroxiredoxin 2 (PRX-2) is the third most abundant protein in RBCs and this study examined the potential role of PRX-2 in inducing brain injury in rats. First, adult male Sprague-Dawley rats had an intracaudate injection of lysed RBCs or saline. Brains were harvested at 1 h to measure PRX-2 levels. Second, rats had an intracaudate injection of either recombinant PRX-2, heat-inactivated PRX-2, or saline. Third, rats had intracaudate co-injection of lysed RBCs with conoidin A, a PRX-2 inhibitor, or vehicle. For the second and third parts of studies, behavioral tests were performed and all rats had magnetic resonance imaging prior to euthanasia for brain immunohistochemistry and Western blotting. We found that brain PRX-2 levels were increased after lysed RBC injection. Intracaudate injection of PRX-2 resulted in blood-brain barrier disruption, brain swelling, neutrophil infiltration, microglia activation, neuronal death, and neurological deficits. Intracerebral injection of lysed RBCs induced brain injury, which was reduced by conoidin A. These results suggest that extracellular PRX-2 released from hematoma can cause brain injury following brain hemorrhage and could be a potential therapeutic target. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

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出版当年[2018]版：

大类 | 1 区

医学

小类 | 1 区神经科学 2 区临床神经病学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区临床神经病学 2 区神经科学

第一作者：

第一作者机构： [1]Department of Neurosurgery, University ofMichigan, R5018 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA [2]Department of Neurology, Beijing Tiantan Hospital, CapitalMedical University, Beijing, China

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通讯机构： [1]Department of Neurosurgery, University ofMichigan, R5018 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA

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Intracerebral Hemorrhage–Induced Brain Injury in Rats: the Role of Extracellular Peroxiredoxin 2

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