机构：[1]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China.临床科室科研平台职能科室耳鼻咽喉头颈外科临床流行病与循证医学中心儿科研究所首都医科大学附属北京儿童医院[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, No.56 Nanlishi Rd., Beijing 100045, China.临床科室职能科室耳鼻咽喉头颈外科临床流行病与循证医学中心首都医科大学附属北京儿童医院[3]Biobank for Clinical Data and Samples in Pediatric, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China.科研平台职能科室临床流行病与循证医学中心儿科研究所首都医科大学附属北京儿童医院[4]State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, Peking Union Medical College and Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Beijing, China.[5]Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing, China.
Background: Papillary thyroid carcinoma (PTC) is one of the most frequent malignancies of the endocrine system, whose mechanisms of pathogenesis, progression and prognosis are still far from being clearly elucidated. Despite an increasing body of evidences highlights ribosome biogenesis regulator homolog (RRS1) as a ribosome biogenesis protein in yeast and plants, little is known about human RRS1 function. Methods: Proliferation, cell cycle and apoptosis of PTC cells were assessed following the knockdown of RRS1 expression though MTT, colony formation assay, and flow cytometry. Then, transcriptome profiling was conducted to explore pathway changes after RRS1 silencing in PTC cells. Receiver operating characteristic curve and Youden's index were performed in twenty-four thyroid carcinoma samples to assess their potential clinical diagnostic value. Results: Firstly, we found that silencing RRS1 significantly reduced cell proliferation, inhibited cell cycle, and promoted apoptosis in PTC cell line. The result also showed that knock-down of RRS1 could up-regulate genes involving apoptosis and metabolism, while, down-regulate genes relative to cell proliferation and blood vessel development. Notably, the present study confirmed the diagnostic value of RRS1 for thyroid carcinoma in both children and adults. Conclusions: In conclusion, these data afford a comprehensive view of a novel function of human RRS1 by promoting cell proliferation and could be a potential indicator for papillary thyroid carcinoma.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81502144, 81472369]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201508]; Beijing Health System Top Level Health Technical Personnel Training Plan [20153079]
第一作者机构：[1]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China.[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, No.56 Nanlishi Rd., Beijing 100045, China.
通讯作者：
通讯机构：[1]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China.[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, No.56 Nanlishi Rd., Beijing 100045, China.[3]Biobank for Clinical Data and Samples in Pediatric, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China.
推荐引用方式(GB/T 7714)：
Chen Feng,Jin Yaqiong,Feng Lin,et al.RRS1 gene expression involved in the progression of papillary thyroid carcinoma[J].CANCER CELL INTERNATIONAL.2018,18(1):-.doi:10.1186/s12935-018-0519-x.
APA：
Chen, Feng,Jin, Yaqiong,Feng, Lin,Zhang, Jie,Tai, Jun...&Ni, Xin.(2018).RRS1 gene expression involved in the progression of papillary thyroid carcinoma.CANCER CELL INTERNATIONAL,18,(1)
MLA：
Chen, Feng,et al."RRS1 gene expression involved in the progression of papillary thyroid carcinoma".CANCER CELL INTERNATIONAL 18..1(2018):-
