Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
机构：[a]Department of Clinical Microbiology, Second Hospital of Hebei Medical University, Hebei Provincial Center for Clinical Laboratories, Shijiazhuang, Hebei, China[b]State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China[c]Public Health College of Hebei Medical University, Shijiazhuang, Hebei, China[d]bioMerieux SA, Marcy-l'Etoile, France[e]bioMerieux, Inc., Hazelwood, Missouri, USA[f]Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China[g]Department of Clinical Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, China首都医科大学附属天坛医院[h]Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA[i]Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York, USA[j]Department of Laboratory Medicine, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei, China.
Clostridium difficile multilocus sequence type 37 (ST37), which mainly corresponds to ribotype 017, has been a dominant genotype circulating in China. In this study, we report the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 204 C. difficile clinical isolates, including 49 ST37 and 155 non-ST37 isolates collected in China and other countries. The distributions of two major protein peaks (m/z 3,242 and 3,286) were significantly different between ST37 and non-ST37 prototype strains and clinical isolates. This difference was reproducible when analysis was performed on different colonies in different runs. This finding was repeated and confirmed by both bioMerieux Vitek MS and Bruker Microflex LT systems on isolates recovered from a variety of geographic regions worldwide. The combination of the two peaks was present in 47 of 49 ST37 isolates, resulting in a sensitivity of 95.9%. In contrast, the peak combination was absent in 153 of 155 non-ST37 isolates, resulting in a specificity of 98.7%. Our results suggest that MALDI-TOF MS is a rapid and reliable tool to identify C. difficile genotype ST37. Work is in progress to characterize the two molecules having peaks at m/z 3,242 and 3,286, which appear to be specific to C. difficile genotype ST37.
基金：
Natural Science Fund of Hebei Province [2013206450]; Memorial Sloan Kettering Cancer Center [SK2012-0595]; bioMerieux, Inc. [SK2012-0595]; NIH/NCIUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [CA008748]
第一作者机构：[a]Department of Clinical Microbiology, Second Hospital of Hebei Medical University, Hebei Provincial Center for Clinical Laboratories, Shijiazhuang, Hebei, China[j]Department of Laboratory Medicine, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei, China.
通讯作者：
通讯机构：[a]Department of Clinical Microbiology, Second Hospital of Hebei Medical University, Hebei Provincial Center for Clinical Laboratories, Shijiazhuang, Hebei, China[h]Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA[i]Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York, USA
推荐引用方式(GB/T 7714)：
Ruxin Li,Di Xiao,Jing Yang,et al.Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry[J].JOURNAL OF CLINICAL MICROBIOLOGY.2018,56(5):-.doi:10.1128/JCM.01990-17.
APA：
Ruxin Li,Di Xiao,Jing Yang,Suju Sun,,Samuel Kaplan,...&Jianhong Zhao.(2018).Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.JOURNAL OF CLINICAL MICROBIOLOGY,56,(5)
MLA：
Ruxin Li,et al."Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry".JOURNAL OF CLINICAL MICROBIOLOGY 56..5(2018):-
医学