Objective: To assess differences in biceps brachii muscle (BBM) stiffness as evaluated by ultrasound shear wave elastography (SWE). Methods: The passive stiffness of the BBM was quantified with shear wave velocity (SWV) measurements obtained from 10 healthy volunteers (5 men and 5 women, mean age 50 years, age range 42-63 years) with the elbow at full extension and 30 degrees flexion in this IRB-approved study. Potential differences between two depths within the muscle, two elbow positions, the two arms, and sexes were assessed by using two-tailed t-test. The reproducibility of SWV measurements was tested by using intraclass correlation coefficient (ICC). Results: Significantly higher passive BBM stiffness was found at full elbow extension compared to 30 of flexion (p <= 0.00006 for both arms). Significantly higher passive stiffness in women was seen for the right arm (p = 0.04 for both elbow positions). Good correlation of shear wave velocity measured at the different depths. The ICC for interobserver and intraobserver variation was high. Conclusions: SWE is a reliable quantitative tool for assessing BBM stiffness, with differences in stiffness based on elbow position demonstrated and based on sex suggested. (C) 2017 Elsevier Inc. All rights reserved.