Objective To characterize carbapenem (CPM)-non-susceptible Klebsiella pneumoniae (K. pneumoniae) and carbape-nemase produced by these strains isolated from Beijing Children's Hospital based on a five-year surveillance. Methods The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonix100 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis. Results In total, 179 strains of CPM-non-susceptible K. pneumoniae were isolated from January, 2010 to December, 2014. The rates of non-susceptible to imipenem and meropenem were 95.0% and 95.6%, respectively. In the 179 strains, 95 (53.1%) strains carried the bla(IMP) gene, and IMP-4 and IMP-8 were detected in 92 (96.8%) and 3 (3.2%) IMP-producing isolates, respectively. 65 (36.3%) strains carried the bla(NDM-1) gene. 6 (3.4%) strains carried the bla(KPC) gene, and KPC-2 were detected in 6 KPC-producing isolates. In addition, New Delhi-Metallo-1 (NDM-1) producing isolates increased from 7.1% to 63.0% in five years and IMP-4 producing isolates decreased from 75.0% to 28.3%. Conclusion High frequencies of multiple resistances to antibiotics were observed in the CPM-non-susceptible K. pneumoniae strains isolated from Beijing Children's Hospital. The production of IMP-4 and NDM-1 metallo-beta-lactamases appears to be an important mechanism for CPM-non-susceptible in K. pneumoniae.
基金：
Beijing Children's Hospital [2012MS08]; Beijing Municipal Science and Technology Project [D131100005313014]
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Dept Lab Med, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Pediat Res Inst, Beijing Childrens Hosp, Key Lab Major Dis Children,Minist Educ, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Dept Lab Med, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Pediat Res Inst, Beijing Childrens Hosp, Key Lab Major Dis Children,Minist Educ, Beijing 100045, Peoples R China;
推荐引用方式(GB/T 7714)：
Dong Fang,Lu Jie,Wang Yan,et al.A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1[J].BIOMEDICAL AND ENVIRONMENTAL SCIENCES.2017,30(8):562-569.doi:10.3967/bes2017.075.
APA：
Dong Fang,Lu Jie,Wang Yan,Shi Jin,Zhen Jing Hui...&Song Wen Qi.(2017).A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,30,(8)
MLA：
Dong Fang,et al."A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 30..8(2017):562-569
