AIM: To explore the feasibility and reliability of CO2 laser soldering on the reconstruction of dura mater in the minipig model. MATERIAL and METHODS: Ten minipigs were divided into 2 groups as Group A (n=5) and Group B (n=5). Bilateral fronto-parietal craniotomy was performed and 2cmx1cm dural defect created under general anesthesia. Then, the defect was repaired with autologous temporalis fascia by CO2 laser soldering. After pressure and watertightness testing, the minipigs of group A were sacrificed immediately. Minipigs in Group B were followed for 4 weeks, with daily monitoring of behavior, food intake, skin incision and neurological condition. Animals of Group B were also subjected to the same tests as group A. Then, they were also sacrificed. The reconstructed area and underlying brain tissue were fixed in paraformaldehyde and submitted for histological analysis. RESULTS: No neural impairment, hydrops or empyema, and no cerebrospinal fluid leak in the dura-fasbia interface were observed in Group B. The mean burst pressures were higher than the mean intracranial crest pressure in groups A and B. This difference was significant (P=0.010, P=0.000, respectively). The physiological intracranial pressure of ten minipigs ranged between 4.53 and 6.47 mmHg. No thermal injury was observed in either group. CONCLUSION: CO2 laser soldering for dural defect reconstruction was feasible and reliable.