机构：[1]Capital Med Univ, Beijing Youan Hosp, Beijing 100069, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Minist Educ, Key Lab Major Dis Children, Beijing, Peoples R China;首都医科大学附属北京儿童医院[3]Capital Med Univ, Beijing Childrens Hosp, Immunol Lab, Beijing Pediat Res Inst, Beijing, Peoples R China;科研平台儿科研究所首都医科大学附属北京儿童医院[4]Chinese Acad Med Sci, MOH Key Lab Syst Biol Pathogens, Beijing 100730, Peoples R China;[5]Chinese Acad Med Sci, AIDS Res Ctr, Inst Pathogen Biol, Beijing 100730, Peoples R China;[6]Peking Union Med Coll, Beijing 100021, Peoples R China
The risk of sexual transmission of HIV is strongly correlated with amounts of genital HIV RNA. Few studies have reported amounts of HIV RNA and HIV DNA in semen in HIV-infected Chinese patients undergoing antiviral treatment (ART). In this observational study, the amounts of HIV RNA and HIV DNA in semen were assessed after six months of ART in HIV-infected Chinese individuals, when HIV RNA was undetectable in blood . This study included 19 HIV-infected Chinese men undergoing ART for six months. Amounts of HIV in paired semen and blood samples were assessed using real-time PCR. The C2-V5 region of the HIV envelope (env) genes was cloned and sequenced and genotype and co-receptor usage predicted based on the sequence. It was found that HIV RNA was undetectable in the plasma of most patients (17/19), whereas HIV RNA could be detected in the semen of most patients (16/19). HIV DNA could be detected in both semen and blood. Genetic diversity of HIV between the seminal and blood compartments was identified. Thus, amounts of HIV RNA and HIV DNA remain high in semen of HIV-infected Chinese patients after six months of ART treatment, even when HIV RNA was undetectable in blood.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China [81371803, 81571973]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7152072]; Beijing Science And Technology Plan [D141100000314004]; National Science and Technology Major Project of China [2015ZX10004801-002-002]; High Levels Of Health Technical Personnel Plan of the Beijing city health system; Development and Application Research of Beijing AIDS Clinical Data and Sample Repository [D131100005313005]
第一作者机构：[1]Capital Med Univ, Beijing Youan Hosp, Beijing 100069, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Youan Hosp, Beijing 100069, Peoples R China;
推荐引用方式(GB/T 7714)：
Du Peiwei,Liu An,Jiao Yanmei,et al.HIV RNA and proviral HIV DNA can be detected in semen after 6 months of antiretroviral therapy although HIV RNA is undetectable in blood[J].MICROBIOLOGY AND IMMUNOLOGY.2016,60(3):187-195.doi:10.1111/1348-0421.12361.
APA：
Du, Peiwei,Liu, An,Jiao, Yanmei,Liu, Cuie,Jiang, Taiyi...&Sun, Lijun.(2016).HIV RNA and proviral HIV DNA can be detected in semen after 6 months of antiretroviral therapy although HIV RNA is undetectable in blood.MICROBIOLOGY AND IMMUNOLOGY,60,(3)
MLA：
Du, Peiwei,et al."HIV RNA and proviral HIV DNA can be detected in semen after 6 months of antiretroviral therapy although HIV RNA is undetectable in blood".MICROBIOLOGY AND IMMUNOLOGY 60..3(2016):187-195
