机构：[1]Univ Virginia, Dept Anesthesiol, Charlottesville, VA 22901 USA;[2]Capital Med Univ, Beijing Tian Tan Hosp, Dept Anaesthesiol, Beijing 100050, Peoples R China
Background: Dexmedetomidine, a sedative agent, provides neuroprotection when administered during or before brain ischaemia. This study was designed to determine whether dexmedetomidine post-treatment induces neuroprotection against subarachnoid haemorrhage (SAH) and the mechanisms for this effect. Methods: Subarachnoid haemorrhage was induced by endovascular perforation to the junction of the right middle and anterior cerebral arteries in adult rats. Dexmedetomidine was applied immediately or 2 h after onset of SAH. Neurological outcome was evaluated 2 days after SAH. Right frontal cortex area 1 was harvested 24 h after SAH for western blotting. Results: Subarachnoid haemorrhage reduced neurological scores and increased brain oedema and blood-brain barrier permeability. These effects were attenuated by dexmedetomidine post-treatment. Neuroprotection by dexmedetomidine was abolished by PD98095, an inhibitor of extracellular signal-regulated kinase (ERK) activation. Phospho-ERK, the activated form of ERK, was increased by dexmedetomidine; this activation was inhibited by PD98095. Conclusions: Dexmedetomidine post-treatment provides neuroprotection against SAH. This effect appears to be mediated by ERK.
基金：
National Institutes of Health, Bethesda, MD, USAUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 GM098308]; International Anaesthesia Research Society, Cleveland, OH, USA (Frontiers in Anaesthesia Research Award); American Heart Association Mid-Atlantic Affiliate by a Grant-in-Aid Baltimore, MD, USA [10GRNT3900019]; University of Virginia, Charlottesville, VA, USA (Robert M. Epstein Professorship endowment)
第一作者机构：[1]Univ Virginia, Dept Anesthesiol, Charlottesville, VA 22901 USA;[2]Capital Med Univ, Beijing Tian Tan Hosp, Dept Anaesthesiol, Beijing 100050, Peoples R China
通讯作者：
通讯机构：[1]Univ Virginia, Dept Anesthesiol, Charlottesville, VA 22901 USA;[2]Capital Med Univ, Beijing Tian Tan Hosp, Dept Anaesthesiol, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Y.,Han R.,Zuo Z..Dexmedetomidine post-treatment induces neuroprotection via activation of extracellular signal-regulated kinase in rats with subarachnoid haemorrhage[J].BRITISH JOURNAL OF ANAESTHESIA.2016,116(3):384-392.doi:10.1093/bja/aev549.
APA：
Wang, Y.,Han, R.&Zuo, Z..(2016).Dexmedetomidine post-treatment induces neuroprotection via activation of extracellular signal-regulated kinase in rats with subarachnoid haemorrhage.BRITISH JOURNAL OF ANAESTHESIA,116,(3)
MLA：
Wang, Y.,et al."Dexmedetomidine post-treatment induces neuroprotection via activation of extracellular signal-regulated kinase in rats with subarachnoid haemorrhage".BRITISH JOURNAL OF ANAESTHESIA 116..3(2016):384-392
医学