Allicin, a sulphur containing compound isolated from the bulb of Allium sativum, confirm the various pharmacological activity such as antioxidant and antiinflammatory effects. The current study was intended to evaluate the effect of allicin on the IL-1β-Induced inflammatory cytokines in Human Osteoarthritis Chondrocytes. The chondrocytes were stimulated with IL-1β in the presence or absence of allicin. Moreover, the western blot analyses was used for the estimation of the nuclear factor-κB (NF-κB), c-Jun N-terminal kinase (JNK), cyclooxygenase-2 (COX-2), inhibitory kappa B (IκBα), extracellular signal-regulated kinase (ERK), iNOS and p38 expression. The level of PGE2 and NO production in the IL-1β treatment were determined via using the ELISA and Griess reagent. In the current study, allicin was found to be significantly inhibited the IL-1β induced iNOS and COX-2 expression in dose dependent manner. On the other hand, allicin in dose-dependent manner causes significant (P < 0.01) reduction of the IL-1β-induced PGE2, NO and collagenase-3 (MMP-13) production. Allicin also showed significant reduction of the IL-1β-induced mitogen-activated protein kinase (MAPK) and NF-κB activation. In conclusion, allicin showed efficient attenuation of the osteoarthritis chondrocytes inflammatory response triggered by IL-1β. The result of the current study confirmed that allicin may be beneficial agent in the management or control of osteoarthritis.
语种：
外文
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区肿瘤学4 区病理学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学4 区病理学
第一作者：
第一作者机构：[1]Department of Orthopaedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China
推荐引用方式(GB/T 7714)：
Wang L,Pan H.-T,Wang B,et al.Allicin reduces IL-1β-induced inflammatory cytokines via attenuating the NF-κB and MMP3 activation in human osteoarthritis chondrocytes model[J].2016,9(10):
APA：
Wang, L,Pan, H.-T,Wang, B,Liu, Y,Fei, K&Han, W.-F.(2016).Allicin reduces IL-1β-induced inflammatory cytokines via attenuating the NF-κB and MMP3 activation in human osteoarthritis chondrocytes model.,9,(10)
MLA：
Wang, L,et al."Allicin reduces IL-1β-induced inflammatory cytokines via attenuating the NF-κB and MMP3 activation in human osteoarthritis chondrocytes model". 9..10(2016)
