Mitochondria are central to the execution of apoptosis, and the Bcl-2 protein family of pro- and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the 3'UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a levels in primary astrocyte cultures and found miR-181a reduction was associated with increased Bcl-2 and Mcl-1 protein levels. Decreased miR-181a levels reduced glucose deprivation induced apoptosis, mitochondrial dysfunction, and loss of mitochondrial membrane potential in astrocytes. (C) 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
基金：
NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [NS053898, GM49831]
第一作者机构：[1]Stanford Univ, Dept Anesthesia, Sch Med, Stanford, CA 94305 USA;
通讯作者：
通讯机构：[1]Stanford Univ, Dept Anesthesia, Sch Med, Stanford, CA 94305 USA;[4]Stanford Univ, Dept Anesthesia, Sch Med, 300 Pasteur Dr,S272A & S290, Stanford, CA 94305 USA
推荐引用方式(GB/T 7714)：
Ouyang Yi-Bing,Lu Yu,Yue Sibiao,et al.miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes[J].MITOCHONDRION.2012,12(2):213-219.doi:10.1016/j.mito.2011.09.001.
APA：
Ouyang, Yi-Bing,Lu, Yu,Yue, Sibiao&Giffard, Rona G..(2012).miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes.MITOCHONDRION,12,(2)
MLA：
Ouyang, Yi-Bing,et al."miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes".MITOCHONDRION 12..2(2012):213-219
