机构：[1]Beijing Neurosurg Inst, Beijing 100050, Peoples R China;[2]Capital Med Univ, Beijing 100069, Peoples R China;[3]Beijing Tiantan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China
Background Adrenocorticotrophin (ACTH)-secreting pituitary adenomas account for approximately 7%-14% of all pituitary adenomas, but its pathogenesis is still enigmatic. This study aimed to explore mechanisms underlying the pathogenesis of ACTH-secreting pituitary adenomas.Methods We used fiber-optic beadarray to examine gene expression in three ACTH-secreting adenomas compared with three normal pituitaries. Four differentially expressed genes from the three ACTH-secreting adenomas and three normal pituitaries were chosen randomly for validation by reverse transcriptase-real time quantitative polymerase chain reaction (RT-qPCR). We then analyzed the differentially expressed gene profile with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway.Results Fiber-optic beadarray analysis showed that the expression of 28 genes and 8 expressed sequence tags (ESTs)were significantly increased and the expression of 412 genes and 31 ESTs were significantly decreased. Bioinformatic and pathway analysis showed that the genes HIGD1B, EPS8, HPGD, DAPK2, and IGFBP3 and the transforming growth factor (TGF)-β signaling pathway and extracellular matrix (ECM)-receptor interaction pathway may play important roles in tumorigenesis and progression of ACTH-secreting pituitary adenomas.Conclusions Our data suggest that numerous aberrantly expressed genes and several pathways are involved in the pathogenesis of ACTH-secreting pituitary adenomas. Fiber-optic beadarray combined with pathway analysis of differential gene expression appears to be a valid method of investigating tumour pathogenesis.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30872651]
JIANG Zhi-quan,GUI Song-bo,ZHANG Ya-zhuo.Differential gene expression by fiber-optic beadarray and pathway in adrenocorticotrophin-secreting pituitary adenomas[J].CHINESE MEDICAL JOURNAL.2010,123(23):3455-3461.doi:10.3760/cma.j.issn.0366-6999.2010.23.015.
APA：
JIANG Zhi-quan,GUI Song-bo&ZHANG Ya-zhuo.(2010).Differential gene expression by fiber-optic beadarray and pathway in adrenocorticotrophin-secreting pituitary adenomas.CHINESE MEDICAL JOURNAL,123,(23)
MLA：
JIANG Zhi-quan,et al."Differential gene expression by fiber-optic beadarray and pathway in adrenocorticotrophin-secreting pituitary adenomas".CHINESE MEDICAL JOURNAL 123..23(2010):3455-3461
