Background: Regulatory T cells and immunosuppressive cytokines, such as IL-10 and TGF-beta(1), may have a role in clinically effective allergen-specific immunotherapy. IL-10-secreting regulatory T cells have emerged as potential mediators of immune tolerance in numerous murine models of immunopathology. The aim of this study was to evaluate the frequency and function of regulatory T cells in the response to house dust mite (HDM) immunotherapy. Methods: PBMCs were isolated from 27 HDM-allergic asthmatic children who underwent immunotherapy for 1.5-2 years (SIT group) and from 27 matched treated asthmatic children allergic to HDM (asthma group). After 48 h of in vitro stimulation with HDM extracts, regulatory T cells were measured by flow cytometry. Production of IL-4, IFN-gamma and TGF-beta(1) in supernatants from allergen-stimulated cultures and the PBMC proliferations were measured by ELISA. Sera were tested for allergen-specific IgE using the ImmunoCAP 100 assay. Results: Patients undergoing immunotherapy produced significantly more IL-10 and showed a significant reduction in proliferation induced by HDM extract compared with the asthma group. In cultures stimulated with HDM extract, the amounts of IL-4 and TGF-beta were lower and the amounts of IFN-gamma were higher in the SIT group compared with the asthma group. Conclusion: There is a functional, but quantitative, insufficiency of Treg cells in allergic asthmatic children, which was reversed in SIT-treated children. SIT can up-regulate the function of CD4(+)CD25(+)Foxp3(+) Treg cells. Copyright (C) 2010 S. Karger AG, Basel
基金：
Beijing Municipal Science & Technology CommissionBeijing Municipal Science & Technology Commission [2006B07]
第一作者机构：[1]Capital Med Univ, Asthma Ctr, Beijing Childrens Hosp, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Asthma Ctr, Beijing Childrens Hosp, Beijing 100045, Peoples R China;[2]Capital Med Univ, Asthma Ctr, Beijing Childrens Hosp, 56 S Lishi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Wei Wang,Liu Yongge,Wang Yonghong,et al.Induction of CD4(+)CD25(+)Foxp3(+)IL-10(+) T Cells in HDM-Allergic Asthmatic Children with or without SIT[J].INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY.2010,153(1):19-26.doi:10.1159/000301575.
APA：
Wei, Wang,Liu, Yongge,Wang, Yonghong,Zhao, Yuhong,He, Jianxin...&Shen, Kunling.(2010).Induction of CD4(+)CD25(+)Foxp3(+)IL-10(+) T Cells in HDM-Allergic Asthmatic Children with or without SIT.INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY,153,(1)
MLA：
Wei, Wang,et al."Induction of CD4(+)CD25(+)Foxp3(+)IL-10(+) T Cells in HDM-Allergic Asthmatic Children with or without SIT".INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 153..1(2010):19-26
