机构：[a]Beijing Neurosurgical Institute, Capital University of Medical Sciences, Beijing 100050, China研究所北京市神经外科研究所首都医科大学附属天坛医院[b]Department of Neuroradiology, Beijing Tiantan Hospital, Beijing 100050, China重点科室医技科室放射科放射科首都医科大学附属天坛医院
Objective. To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age. Methods. Enucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry. Results. bFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P < 0.05). Conclusion bFGF might play an important role in the proliferation of residual human cataract LECs after cataract surgery.
Ren A,Gao P,Sun Y.Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells[J].CHINESE MEDICAL JOURNAL.2002,115(2):
APA：
Ren, A,Gao, P&Sun, Y.(2002).Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells.CHINESE MEDICAL JOURNAL,115,(2)
MLA：
Ren, A,et al."Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells".CHINESE MEDICAL JOURNAL 115..2(2002)
